EC - Precorrin-6B C5,15-methyltransferase (decarboxylating)

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
precorrin-6B C5,15-methyltransferase (decarboxylating)
Other names:
precorrin-6 methyltransferase
precorrin-6Y methylase
precorrin-6Y C5,15-methyltransferase (decarboxylating)
cobL (gene name)
Systematic name:
S-adenosyl-L-methionine:1-precorrin-6B C5,15-methyltransferase (C-12-decarboxylating)



The enzyme participates in the aerobic (late cobalt insertion) adenosylcobalamin biosynthesis pathway. The enzyme from the bacterium Pseudomonas denitrificans is a fusion protein with two active sites; one catalyses the methylation at C-15 followed by decarboxylation of the C-12 acetate side chain, while the other catalyses the methylation at C-5. The corresponding activities in the anaerobic adenosylcobalamin biosynthesis pathway are catalysed by EC and EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0046025
CAS Registry Number: 162995-22-4


  1. Blanche, F., Famechon, A., Thibaut, D., Debussche, L., Cameron, B. and Crouzet, J.
    Biosynthesis of vitamin B12 in Pseudomonas denitrificans: the biosynthetic sequence from precorrin-6Y to precorrin-8X is catalyzed by the cobL gene product.
    J. Bacteriol. 174 : 1050-1052 (1992). [PMID: 1732195]
  2. Deery, E., Schroeder, S., Lawrence, A. D., Taylor, S. L., Seyedarabi, A., Waterman, J., Wilson, K. S., Brown, D., Geeves, M. A., Howard, M. J., Pickersgill, R. W., Warren, M. J.
    An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis.
    Nat. Chem. Biol. 8 : 933-940 (2012). [PMID: 23042036]

[EC created 1999, modified 2013]