EC 126.96.36.199 - Thiosulfate dehydrogenase
IntEnz Enzyme Nomenclature
tsdA (gene name)
20549 [IUBMB]2[Fe(III)cytochrome c][Fe(III)cytochrome c]GENERIC:14399Is ROOT: no
ROOT compound: GENERIC:9753Number of residues: 12thiosulfatethiosulfateName origin: UniProt - CHECKED (C)Formula: HO3S2
Charge: -1ChEBI compound status: CHECKED (C)=2[Fe(II)cytochrome c][Fe(II)cytochrome c]GENERIC:10350Is ROOT: no
ROOT compound: GENERIC:9753Number of residues: 12H+H(+)Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C)
The enzyme catalyses the reversible formation of a sulfur-sulfur bond between the sulfane atoms of two thiosulfate molecules, yielding tetrathionate and releasing two electrons. In many bacterial species the enzyme is a diheme c-type cytochrome. In a number of organisms, including Thiomonas intermedia and Sideroxydans lithotrophicus, a second diheme cytochrome (TsdB) acts as the electron acceptor. However, some organisms, such as Allochromatium vinosum, lack TsdB. The electron acceptor in these organisms may be the high-potential iron-sulfur protein (HiPIP).
Links to other databases
Cellular location and partial purification of the 'thiosulphate-oxidizing enzyme' and 'trithionate hydrolase' from Thiobacillus tepidarius.J. Gen. Microbiol. 134: 877-885 (1988).
A high-potential nonheme iron protein (HiPIP)-linked, thiosulfate-oxidizing enzyme derived from Chromatium vinosum.Curr. Microbiol. 3: 117-120 (1979).
Tetrathionate stimulated growth of Campylobacter jejuni identifies a new type of bi-functional tetrathionate reductase (TsdA) that is widely distributed in bacteria.Mol. Microbiol. 88: 173-188 (2013). [PMID: 23421726]
Thiosulfate dehydrogenase (TsdA) from Allochromatium vinosum: structural and functional insights into thiosulfate oxidation.J. Biol. Chem. 290: 9222-9238 (2015). [PMID: 25673691]
Electron Accepting Units of the Diheme Cytochrome c TsdA, a Bifunctional Thiosulfate Dehydrogenase/Tetrathionate Reductase.J. Biol. Chem. 291: 24804-24818 (2016). [PMID: 27694441]
[EC 188.8.131.52 created 1990]