EC - Nitrate reductase (quinone)

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IntEnz Enzyme Nomenclature


Accepted name:
nitrate reductase (quinone)
Other names:
nitrate reductase A
nitrate reductase Z
quinol/nitrate oxidoreductase
quinol-nitrate oxidoreductase
quinol:nitrate oxidoreductase
dissimilatory nitrate reductase
Systematic name:
nitrite:quinone oxidoreductase




A membrane-bound enzyme which supports anaerobic respiration on nitrate under anaerobic conditions and in the presence of nitrate. Contains the bicyclic form of the molybdo-bis(molybdopterin guanine dinucleotide) cofactor, iron-sulfur clusters and heme b. Escherichia coli expresses two forms NarA and NarZ, both being comprised of three subunits.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00392
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (20) [show] [UniProt]


  1. Enoch, H. G., Lester, R. L.
    The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli.
    Biochem. Biophys. Res. Commun. 61 : 1234-1241 (1974). [PMID: 4616697]
  2. Bertero, M. G., Rothery, R. A., Palak, M., Hou, C., Lim, D., Blasco, F., Weiner, J. H., Strynadka, N. C.
    Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A.
    Nat. Struct. Biol. 10 : 681-687 (2003). [PMID: 12910261]
  3. Lanciano, P., Magalon, A., Bertrand, P., Guigliarelli, B., Grimaldi, S.
    High-stability semiquinone intermediate in nitrate reductase A (NarGHI) from Escherichia coli is located in a quinol oxidation site close to heme bD.
    Biochemistry 46 : 5323-5329 (2007). [PMID: 17439244]
  4. Bertero, M. G., Rothery, R. A., Boroumand, N., Palak, M., Blasco, F., Ginet, N., Weiner, J. H., Strynadka, N. C.
    Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A.
    J. Biol. Chem. 280 : 14836-14843 (2005). [PMID: 15615728]
  5. Bonnefoy, V., Demoss, J. A.
    Nitrate reductases in Escherichia coli.
    Antonie Van Leeuwenhoek 66 : 47-56 (1994). [PMID: 7747940]
  6. Guigliarelli, B., Asso, M., More, C., Augier, V., Blasco, F., Pommier, J., Giordano, G., Bertrand, P.
    EPR and redox characterization of iron-sulfur centers in nitrate reductases A and Z from Escherichia coli. Evidence for a high-potential and a low-potential class and their relevance in the electron-transfer mechanism.
    Eur. J. Biochem. 207 : 61-68 (1992). [PMID: 1321049]

[EC created 2010]