EC - Nitroalkane oxidase

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IntEnz Enzyme Nomenclature


Accepted name:
nitroalkane oxidase
Other names:
nitroethane reductase
nitroethane oxidase
nitroethane:oxygen oxidoreductase
Systematic name:
nitroalkane:oxygen oxidoreductase




Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC, 2-nitropropane dioxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052664 , GO:0050141
CAS Registry Numbers: 65802-82-6 9029-36-1


  1. Little, H.N.
    Oxidation of nitroethane by extracts from Neurospora.
    J. Biol. Chem. 193 : 347-358 (1951). [PMID: 14907722]
  2. Kido, T., Hashizume, K. and Soda, K.
    Purification and properties of nitroalkane oxidase from Fusarium oxysporum.
    J. Bacteriol. 133 : 53-58 (1978). [PMID: 22538]
  3. Daubner, S.C., Gadda, G., Valley, M.P and Fitzpatrick, P.F.
    Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new member of the acyl-CoA dehydrogenase superfamily.
    Proc. Natl. Acad. Sci. USA 99 : 2702-2707 (2002). [PMID: 11867731]
  4. Fitzpatrick, P.F., Orville, A.M., Nagpal, A. and Valley, M.P.
    Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to acyl-CoA dehydrogenase.
    Arch. Biochem. Biophys. 433 : 157-165 (2005). [PMID: 15581574]
  5. Valley, M.P., Tichy, S.E. and Fitzpatrick, P.F.
    Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase.
    J. Am. Chem. Soc. 127 : 2062-2066 (2005). [PMID: 15713081]

[EC created 1961, modified 2006, modified 2009]