EC 1 - Oxidoreductases
EC 1.5 - Acting on the CH-NH group of donors
EC 1.5.99 - With other acceptors
EC 1.5.99.4 - Nicotine dehydrogenase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.5.99.4
Names
Accepted name:
nicotine dehydrogenase
Other
names:
D-nicotine oxidase
nicotine oxidase
nicotine:(acceptor) 6-oxidoreductase (hydroxylating)
L-nicotine oxidase
nicotine oxidase
nicotine:(acceptor) 6-oxidoreductase (hydroxylating)
L-nicotine oxidase
Systematic name:
nicotine:acceptor 6-oxidoreductase (hydroxylating)
Reaction
- (S)-nicotine + acceptor + H2O = (S)-6-hydroxynicotine + reduced acceptor
Cofactor
Comments:
A metalloprotein (FMN). The enzyme can act on both the naturally found (S)-enantiomer and the synthetic (R)-enantiomer of nicotine, with retention of configuration in both cases [4].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
UM-BBD
,
UniPathway
Gene Ontology:
GO:0018535
CAS Registry Number:
37256-31-8
References
-
The bacterial oxidation of nicotinic acid.J. Biol. Chem. 228 : 923-945 (1957). [PMID: 13475371]
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Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.Biochim. Biophys. Acta 105 : 313-324 (1965). [PMID: 5849820]
-
The purification and properties of nicotine oxidase.Biochim. Biophys. Acta 139 : 56-68 (1967). [PMID: 4962139]
-
The bacterial oxidation of nicotine. II. The isolation of the first oxidative product and its identification as (1)-6-hydroxynicotine.J. Biol. Chem. 234 : 156-160 (1959). [PMID: 13610912]
[EC 1.5.99.4 created 1972]