EC - Nicotine dehydrogenase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
nicotine dehydrogenase
Other names:
D-nicotine oxidase
nicotine oxidase
nicotine:(acceptor) 6-oxidoreductase (hydroxylating)
L-nicotine oxidase
Systematic name:
nicotine:acceptor 6-oxidoreductase (hydroxylating)




A metalloprotein (FMN). The enzyme can act on both the naturally found (S)-enantiomer and the synthetic (R)-enantiomer of nicotine, with retention of configuration in both cases [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018535
CAS Registry Number: 37256-31-8


  1. Behrman, E.J. and Stanier, R.Y.
    The bacterial oxidation of nicotinic acid.
    J. Biol. Chem. 228 : 923-945 (1957). [PMID: 13475371]
  2. Decker, K. and Bleeg, H.
    Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.
    Biochim. Biophys. Acta 105 : 313-324 (1965). [PMID: 5849820]
  3. Hochstein, L.I. and Dalton, B.P.
    The purification and properties of nicotine oxidase.
    Biochim. Biophys. Acta 139 : 56-68 (1967). [PMID: 4962139]
  4. Hochstein, L.I. and Rittenberg, S.C.
    The bacterial oxidation of nicotine. II. The isolation of the first oxidative product and its identification as (1)-6-hydroxynicotine.
    J. Biol. Chem. 234 : 156-160 (1959). [PMID: 13610912]

[EC created 1972]