EC - (R)-6-hydroxynicotine oxidase

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IntEnz Enzyme Nomenclature


Accepted name:
(R)-6-hydroxynicotine oxidase
Other names:
6-hydroxy-D-nicotine oxidase
D-6-hydroxynicotine oxidase
Systematic name:
(R)-6-hydroxynicotine:oxygen oxidoreductase




A flavoprotein (FAD). The enzyme, which participates in nicotine degradation, is specific for (R) isomer of 6-hydroxynicotine, derived from the uncommon (R)-nicotine. The bacterium Arthrobacter nicotinovorans, in which this enzyme was originally discovered, has a different enzyme that catalyses a similar reaction with the (S)-isomer (cf. EC, (S)-6-hydroxynicotine oxidase).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Protein domains and families: PROSITE:PDOC00674
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018530 , GO:0019116
CAS Registry Number: 37233-46-8


  1. Decker, K. and Bleeg, H.
    Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.
    Biochim. Biophys. Acta 105: 313-324 (1965). [PMID: 5849820]
  2. Brühmüller, M., Möhler, H.K. and Decker, K.
    Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase.
    Eur. J. Biochem. 29: 143-151 (1972). [PMID: 4628374]
  3. Brandsch, R., Hinkkanen, A. E., Mauch, L., Nagursky, H., Decker, K.
    6-Hydroxy-D-nicotine oxidase of Arthrobacter oxidans. Gene structure of the flavoenzyme and its relationship to 6-hydroxy-L-nicotine oxidase.
    Eur. J. Biochem. 167: 315-320 (1987). [PMID: 3622516]
  4. Schenk, S., Hoelz, A., Krauss, B., Decker, K.
    Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans.
    J. Mol. Biol. 284: 1323-1339 (1998). [PMID: 9878353]
  5. Koetter, J. W., Schulz, G. E.
    Crystal structure of 6-hydroxy-D-nicotine oxidase from Arthrobacter nicotinovorans.
    J. Mol. Biol. 352: 418-428 (2005). [PMID: 16095622]

[EC created 1972, modified 2015]