EC - (S)-6-hydroxynicotine oxidase

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IntEnz Enzyme Nomenclature


Accepted name:
(S)-6-hydroxynicotine oxidase
Other names:
6-hydroxy-L-nicotine oxidase
6-hydroxy-L-nicotine:oxygen oxidoreductase
L-6-hydroxynicotine oxidase
nctB (gene name)
Systematic name:
(S)-6-hydroxynicotine:oxygen oxidoreductase




A flavoprotein (FAD). The enzyme, which participates in nicotine degradation, is specific for the (S) isomer of 6-hydroxynicotine. The bacterium Arthrobacter nicotinovorans, in which this enzyme was originally discovered, has a different enzyme that catalyses a similar reaction with the less common (R)-isomer (cf. EC, (R)-6-hydroxynicotine oxidase).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018531
CAS Registry Number: 37256-29-4


  1. Decker, K. and Bleeg, H.
    Induction and purification of stereospecific nicotine oxidizing enzymes from Arthrobacter oxidans.
    Biochim. Biophys. Acta 105 : 313-324 (1965). [PMID: 5849820]
  2. Dai, V.D., Decker, K. and Sund, H.
    Purification and properties of L-6-hydroxynicotine oxidase.
    Eur. J. Biochem. 4 : 95-102 (1968). [PMID: 5646150]
  3. Schenk, S., Hoelz, A., Krauss, B., Decker, K.
    Gene structures and properties of enzymes of the plasmid-encoded nicotine catabolism of Arthrobacter nicotinovorans.
    J. Mol. Biol. 284 : 1323-1339 (1998). [PMID: 9878353]
  4. Qiu, J., Wei, Y., Ma, Y., Wen, R., Wen, Y., Liu, W.
    A novel (S)-6-hydroxynicotine oxidase gene from Shinella sp. strain HZN7.
    Appl. Environ. Microbiol. 80 : 5552-5560 (2014). [PMID: 25002425]

[EC created 1972, modified 2015]