EC - Sarcosine oxidasee (formaldehyde-forming)

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IntEnz Enzyme Nomenclature


Accepted name:
sarcosine oxidasee (formaldehyde-forming)
Other names:
sarcosine oxidasee [ambiguous]
monomeric sarcosine oxidase
Systematic name:
sarcosine:oxygen oxidoreductase (demethylating)




The enzyme, reported from bacteria and fungi, catalyses the oxidative demethylation of sarcosine. It contains a FAD cofactor bound to an L-cysteine residue. cf. EC 1.5.3.l, sarcosine oxidase (5,10-methylenetetrahydrofolate-forming).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008115
CAS Registry Number: 9029-22-5
UniProtKB/Swiss-Prot: (24) [show] [UniProt]


  1. Mori, N., Sano, M., Tani, Y. and Yamada, H.
    Purification and propertie of sarcosine oxidase from Cylindrocarpon didymum M-1.
    Agric. Biol. Chem. 44 : 1391-1397 (1980).
  2. Nishiya, Y. and Imanaka, T.
    Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826.
    J. Ferment. Bioeng. 75 : 239-244 (1993).
  3. Trickey, P., Wagner, M. A., Jorns, M. S., Mathews, F. S.
    Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme.
    Structure 7 : 331-345 (1999). [PMID: 10368302]
  4. Wagner, M. A., Trickey, P., Chen, Z. W., Mathews, F. S., Jorns, M. S.
    Monomeric sarcosine oxidase: 1. Flavin reactivity and active site binding determinants.
    Biochemistry 39 : 8813-8824 (2000). [PMID: 10913292]
  5. Zhao, G., Bruckner, R. C., Jorns, M. S.
    Identification of the oxygen activation site in monomeric sarcosine oxidase: role of Lys265 in catalysis.
    Biochemistry 47 : 9124-9135 (2008). [PMID: 18693755]
  6. Jorns, M. S., Chen, Z. W., Mathews, F. S.
    Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase .
    Biochemistry 49 : 3631-3639 (2010). [PMID: 20353187]
  7. Bucci, A., Yu, T. Q., Vanden-Eijnden, E., Abrams, C. F.
    Kinetics of O2 Entry and Exit in Monomeric Sarcosine Oxidase via Markovian Milestoning Molecular Dynamics.
    J Chem Theory Comput 12 : 2964-2972 (2016). [PMID: 27168219]

[EC created 1961]