EC 1 - Oxidoreductases
EC 1.5 - Acting on the CH-NH group of donors
EC 1.5.3 - With oxygen as acceptor
EC 1.5.3.1 - Sarcosine oxidasee (formaldehyde-forming)
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.5.3.1
Names
Accepted name:
sarcosine oxidasee (formaldehyde-forming)
Other
names:
sarcosine oxidasee
[ambiguous]
monomeric sarcosine oxidase
MSOX
monomeric sarcosine oxidase
MSOX
Systematic name:
sarcosine:oxygen oxidoreductase (demethylating)
Reaction
- sarcosine + H2O + O2 = glycine + formaldehyde + H2O2
Cofactor
Comments:
The enzyme, reported from bacteria and fungi, catalyses the oxidative demethylation of sarcosine. It contains a FAD cofactor bound to an L-cysteine residue. cf. EC 1.5.3.l, sarcosine oxidase (5,10-methylenetetrahydrofolate-forming).
Links to other databases
Gene Ontology:
GO:0008115
CAS Registry Number:
9029-22-5
References
-
Purification and propertie of sarcosine oxidase from Cylindrocarpon didymum M-1.Agric. Biol. Chem. 44 : 1391-1397 (1980).
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Cloning and sequencing of the sarcosine oxidase gene from Arthrobacter sp. TE1826.J. Ferment. Bioeng. 75 : 239-244 (1993).
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Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme.Structure 7 : 331-345 (1999). [PMID: 10368302]
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Monomeric sarcosine oxidase: 1. Flavin reactivity and active site binding determinants.Biochemistry 39 : 8813-8824 (2000). [PMID: 10913292]
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Identification of the oxygen activation site in monomeric sarcosine oxidase: role of Lys265 in catalysis.Biochemistry 47 : 9124-9135 (2008). [PMID: 18693755]
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Structural characterization of mutations at the oxygen activation site in monomeric sarcosine oxidase .Biochemistry 49 : 3631-3639 (2010). [PMID: 20353187]
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Kinetics of O2 Entry and Exit in Monomeric Sarcosine Oxidase via Markovian Milestoning Molecular Dynamics.J Chem Theory Comput 12 : 2964-2972 (2016). [PMID: 27168219]
[EC 1.5.3.1 created 1961]