EC - Methylenetetrahydrofolate dehydrogenase (NADP+)

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IntEnz Enzyme Nomenclature


Accepted name:
methylenetetrahydrofolate dehydrogenase (NADP+)
Other names:
5,10-methylenetetrahydrofolate dehydrogenase
5,10-methylenetetrahydrofolate:NADP oxidoreductase
N5,N10-methylenetetrahydrofolate dehydrogenase
methylenetetrahydrofolate dehydrogenase
methylenetetrahydrofolate dehydrogenase (NADP)
Systematic name:
5,10-methylenetetrahydrofolate:NADP+ oxidoreductase



In eukaryotes, occurs as a trifunctional enzyme also having methenyl-tetrahydrofolate cyclohydrolase (EC and formyltetrahydrofolate synthase (EC activity. In some prokaryotes occurs as a bifunctional enzyme also having methenyltetrahydrofolate cyclohydrolase activity (EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , NIST 74 , UniPathway
Protein domains and families: PROSITE:PDOC00616
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004488 , GO:0004486
CAS Registry Number: 9029-14-5
UniProtKB/Swiss-Prot: (827) [show] [UniProt]


  1. Hatefi, Y., Osborn, M.J., Kay, L.D. and Huennekens, F.M.
    Hydroxymethyl tetrahydrofolic dehydrogenase.
    J. Biol. Chem. 227 : 637-647 (1957). [PMID: 13462986]
  2. Osborn, M.J. and Huennekens, F.M.
    Participation of anhydroleucovorin in the hydroxymethyl tetrahydrofolic dehydrogenase system.
    Biochim. Biophys. Acta 26 : 646-647 (1957). [PMID: 13499428]
  3. Ramasastri, B.V. and Blakley, R.L.
    5,10-Methylenetetrahydrofolic acid dehydrogenase from Bakers' yeast. I. Partial purification and some properties.
    J. Biol. Chem. 237 : 1982-1988 (1962). [PMID: 14490085]
  4. Yeh, Y.-C. and Greenberg, D.M.
    Purification and properties of N5, N10-methylenetetrahydrofolate dehydrogenase of calf thymus.
    Biochim. Biophys. Acta 105 : 279-291 (1965). [PMID: 4379024]

[EC created 1961]