EC - Riboflavin reductase [NAD(P)H]

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IntEnz Enzyme Nomenclature


Accepted name:
riboflavin reductase [NAD(P)H]
Other names:
NAD(P)H-FMN reductase [ambiguous]
NAD(P)H-dependent FMN reductase [ambiguous]
NAD(P)H:FMN oxidoreductase
NAD(P)H:flavin oxidoreductase
NAD(P)H2 dehydrogenase (FMN) [ambiguous]
NAD(P)H2:FMN oxidoreductase [ambiguous]
riboflavin mononucleotide reductase [ambiguous]
flavine mononucleotide reductase [ambiguous]
riboflavin mononucleotide (reduced nicotinamide adenine dinucleotide (phosphate)) reductase
flavin mononucleotide reductase [ambiguous]
riboflavine mononucleotide reductase [ambiguous]
Systematic name:
riboflavin:NAD(P)+ oxidoreductase



Catalyses the reduction of soluble flavins by reduced pyridine nucleotides. Highest activity with riboflavin. When NADH is used as acceptor, the enzyme can also utilize FMN and FAD as substrates, with lower activity than riboflavin. When NADPH is used as acceptor, the enzyme has a very low activity with FMN and no activity with FAD [1].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052875 , GO:0042602


  1. Fontecave, M., Eliasson, R., Reichard, P.
    NAD(P)H:flavin oxidoreductase of Escherichia coli. A ferric iron reductase participating in the generation of the free radical of ribonucleotide reductase.
    J. Biol. Chem. 262 : 12325-12331 (1987). [PMID: 3305505]
  2. Spyrou, G., Haggård-Ljungquist, E., Krook, M., Jörnvall, H., Nilsson, E., Reichard, P.
    Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.
    J. Bacteriol. 173 : 3673-3679 (1991). [PMID: 2050627]
  3. Ingelman, M., Ramaswamy, S., Niviere, V., Fontecave, M., Eklund, H.
    Crystal structure of NAD(P)H:flavin oxidoreductase from Escherichia coli.
    Biochemistry 38 : 7040-7049 (1999). [PMID: 10353815]

[EC created 2011]