EC - FMN reductase (NADPH)

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
FMN reductase (NADPH)
Other names:
flavin reductase P
Systematic name:
FMNH2:NADP+ oxidoreductase




The enzymes from bioluminescent bacteria contain FMN [4], while the enzyme from Escherichia coli does not [8]. The enzyme often forms a two-component system with monooxygenases such as luciferase. Unlike EC, this enzyme does not use NADH as acceptor [1,2]. While FMN is the preferred substrate, the enzyme can also use FAD and riboflavin with lower activity [3,6,8].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052873


  1. Gerlo, E., Charlier, J.
    Identification of NADH-specific and NADPH-specific FMN reductases in Beneckea harveyi.
    Eur. J. Biochem. 57 : 461-467 (1975). [PMID: 1175652]
  2. Jablonski, E., DeLuca, M.
    Purification and properties of the NADH and NADPH specific FMN oxidoreductases from Beneckea harveyi.
    Biochemistry 16 : 2932-2936 (1977). [PMID: 880288]
  3. Jablonski, E., DeLuca, M.
    Studies of the control of luminescence in Beneckea harveyi: properties of the NADH and NADPH:FMN oxidoreductases.
    Biochemistry 17 : 672-678 (1978). [PMID: 23827]
  4. Lei, B., Liu, M., Huang, S., Tu, S. C.
    Vibrio harveyi NADPH-flavin oxidoreductase: cloning, sequencing and overexpression of the gene and purification and characterization of the cloned enzyme.
    J. Bacteriol. 176 : 3552-3558 (1994). [PMID: 8206832]
  5. Tanner, J. J., Lei, B., Tu, S. C., Krause, K. L.
    Flavin reductase P: structure of a dimeric enzyme that reduces flavin.
    Biochemistry 35 : 13531-13539 (1996). [PMID: 8885832]
  6. Liu, M., Lei, B., Ding, Q., Lee, J. C., Tu, S. C.
    Vibrio harveyi NADPH:FMN oxidoreductase: preparation and characterization of the apoenzyme and monomer-dimer equilibrium.
    Arch. Biochem. Biophys. 337 : 89-95 (1997). [PMID: 8990272]
  7. Lei, B., Tu, S. C.
    Mechanism of reduced flavin transfer from Vibrio harveyi NADPH-FMN oxidoreductase to luciferase.
    Biochemistry 37 : 14623-14629 (1998). [PMID: 9772191]
  8. Eichhorn, E., van der Ploeg, J. R., Leisinger, T.
    Characterization of a two-component alkanesulfonate monooxygenase from Escherichia coli.
    J. Biol. Chem. 274 : 26639-26646 (1999). [PMID: 10480865]

[EC created 2011]