IntEnz

EC 1.5.1.3 - Dihydrofolate reductase

IntEnz Enzyme Nomenclature
EC 1.5.1.3

Names

Reaction

• 15009 [IUBMB]
  (6S)-5,6,7,8-tetrahydrofolate

  (6S)-5,6,7,8-tetrahydrofolate

  Name origin: UniProt - CHECKED (C)

  CHEBI:57453

  Formula: C19H21N7O6
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  NADP⁺

  NADP(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:58349

  Formula: C21H25N7O17P3
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  =

  7,8-dihydrofolate

  7,8-dihydrofolate

  Name origin: UniProt - CHECKED (C)

  CHEBI:57451

  Formula: C19H19N7O6
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  NADPH

  NADPH

  Name origin: UniProt - CHECKED (C)

  CHEBI:57783

  Formula: C21H26N7O17P3
  Charge: -4

  ChEBI compound status: CHECKED (C)

  

Comments:

The enzyme from animals and some micro-organisms also slowly reduces folate to 5,6,7,8-tetrahydrofolate.

Links to other databases

References

1. Blakley, R.L. and McDougall, B.M.
   Dihydrofolic reductase from Streptococcus faecalis R.
   J. Biol. Chem. 236: 1163 (1961).
2. Bolin, J.T., Filman, D.J., Matthews, D.A. and Kraut, J.
   Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 Å resolution. I. General features and binding of methotrexate.
   J. Biol. Chem. 257: 13650-13662 (1982). [PMID: 6815178]
3. Kaufman, B.T. and Gardiner, R.C.
   Studies on dihydrofolic reductase. I. Purification and properties of dihydrofolic reductase from chicken liver.
   J. Biol. Chem. 241: 1319-1328 (1966). [PMID: 4379915]
4. Young, I.G. and Gibson, F.
   Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli.
   Biochim. Biophys. Acta 177: 401-411 (1969). [PMID: 4306838]

[EC 1.5.1.3 created 1961, modified 1976 (EC 1.5.1.4 created 1961, incorporated 1976)]