EC 188.8.131.52 - Methylenetetrahydrofolate reductase [NAD(P)H]
IntEnz Enzyme Nomenclature
- 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+
A flavoprotein (FAD). The enzyme catalyses the reversible conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, playing an important role in folate metabolism by regulating the distribution of one-carbon moieties between cellular methylation reactions and nucleic acid synthesis. This enzyme, characterized from Protozoan parasites of the genus Leishmania, is unique among similar characterized eukaryotic enzymes in that it lacks the C-terminal allosteric regulatory domain (allowing it to catalyse a reversible reaction) and uses NADH and NADPH with equal efficiency under physiological conditions. cf. EC 184.108.40.206, methylenetetrahydrofolate reductase (NADPH), EC 220.127.116.11, methylenetetrahydrofolate reductase (NADH), and EC 18.104.22.168, methylenetetrahydrofolate reductase (ferredoxin).
Links to other databases
Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence.J Biol Chem 281 : 38150-38158 (2006). [PMID: 17032644]
[EC 22.214.171.124 created 1978 as EC 126.96.36.199, transferred 1984 to EC 188.8.131.52 (EC 184.108.40.206 incorporated 2005), modified 2005, modified 2021]