IntEnz

EC 1.3.8.2 - 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)

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IntEnz Enzyme Nomenclature
EC 1.3.8.2

Names

Accepted name:

4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)

Other names:

dehydrosqualene desaturase [ambiguous]
CrtN [ambiguous]
4,4'-diapophytoene:FAD oxidoreductase [ambiguous]
15-cis-4,4'-diapophytoene:FAD oxidoreductase
4,4'-diapophytoene desaturase [ambiguous]

Systematic name:

15-cis-4,4'-diapophytoene:FAD oxidoreductase (4,4'-diapolycopene-forming)

Reactions

31391 [IUBMB]

15-cis-4,4'-diapophytoene

15-cis-4,4'-diapophytoene

Name origin: UniProt - CHECKED (C)

CHEBI:62738

Formula: C30H48
Charge: 0

ChEBI compound status: CHECKED (C)

+
4

FAD

FAD

Name origin: UniProt - CHECKED (C)

CHEBI:57692

Formula: C27H30N9O15P2
Charge: -3

ChEBI compound status: CHECKED (C)

+
4

H⁺

H(+)

Name origin: UniProt - CHECKED (C)

CHEBI:15378

Formula: H
Charge: 1

ChEBI compound status: CHECKED (C)

=

all-trans-4,4'-diapolycopene

all-trans-4,4'-diapolycopene

Name origin: UniProt - CHECKED (C)

CHEBI:62449

Formula: C30H40
Charge: 0

ChEBI compound status: CHECKED (C)

+
4

FADH₂

FADH2

Name origin: UniProt - CHECKED (C)

CHEBI:58307

Formula: C27H33N9O15P2
Charge: -2

ChEBI compound status: CHECKED (C)
42800

15-cis-4,4'-diapophytoene

15-cis-4,4'-diapophytoene

Name origin: UniProt - CHECKED (C)

CHEBI:62738

Formula: C30H48
Charge: 0

ChEBI compound status: CHECKED (C)

+
3

FAD

FAD

Name origin: UniProt - CHECKED (C)

CHEBI:57692

Formula: C27H30N9O15P2
Charge: -3

ChEBI compound status: CHECKED (C)

+
3

H⁺

H(+)

Name origin: UniProt - CHECKED (C)

CHEBI:15378

Formula: H
Charge: 1

ChEBI compound status: CHECKED (C)

=

all-trans-4,4'-diaponeurosporene

all-trans-4,4'-diaponeurosporene

Name origin: UniProt - CHECKED (C)

CHEBI:62743

Formula: C30H42
Charge: 0

ChEBI compound status: CHECKED (C)

+
3

FADH₂

FADH2

Name origin: UniProt - CHECKED (C)

CHEBI:58307

Formula: C27H33N9O15P2
Charge: -2

ChEBI compound status: CHECKED (C)

Comments:

The enzyme catalyses four successive dehydrogenations, resulting in production of 4,4'-diapolycopene. While the enzyme from Staphylococcus aureus was only shown to produce 4,4'-diaponeurosporene in vivo [4], it is able to catalyse the last reaction in vitro [5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway

Structural data: CSA , EC2PDB

Gene Ontology: GO:0102223

UniProtKB/Swiss-Prot:

Q4VKV1

CRTN_METSP

References

Wieland, B., Feil, C., Gloria-Maercker, E., Thumm, G., Lechner, M., Bravo, J. M., Poralla, K., Gotz, F.

Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureus.

J. Bacteriol. 176: 7719-7726 (1994). [PMID: 8002598]
Raisig, A., Sandmann, G.

4,4'-diapophytoene desaturase: catalytic properties of an enzyme from the C₃₀ carotenoid pathway of Staphylococcus aureus.

J. Bacteriol. 181: 6184-6187 (1999). [PMID: 10498735]
Raisig, A., Sandmann, G.

Functional properties of diapophytoene and related desaturases of C₃₀ and C₄₀ carotenoid biosynthetic pathways.

Biochim. Biophys. Acta 1533: 164-170 (2001). [PMID: 11566453]
Tao, L., Schenzle, A., Odom, J. M., Cheng, Q.

Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde.

Appl. Environ. Microbiol. 71: 3294-3301 (2005). [PMID: 15933032]
Yoshida, K., Ueda, S., Maeda, I.

Carotenoid production in Bacillus subtilis achieved by metabolic engineering.

Biotechnol. Lett. 31: 1789-1793 (2009). [PMID: 19618272]

[EC 1.3.8.2 created 2011, modified 2011]

EC 1 - Oxidoreductases