EC 1 - Oxidoreductases
EC 1.3 - Acting on the CH-CH group of donors
EC 1.3.8 - With a flavin as acceptor
EC 1.3.8.2 - 4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.3.8.2
Names
Accepted name:
4,4'-diapophytoene desaturase (4,4'-diapolycopene-forming)
Other
names:
dehydrosqualene desaturase
[ambiguous]
CrtN [ambiguous]
4,4'-diapophytoene:FAD oxidoreductase [ambiguous]
15-cis-4,4'-diapophytoene:FAD oxidoreductase
4,4'-diapophytoene desaturase [ambiguous]
CrtN [ambiguous]
4,4'-diapophytoene:FAD oxidoreductase [ambiguous]
15-cis-4,4'-diapophytoene:FAD oxidoreductase
4,4'-diapophytoene desaturase [ambiguous]
Systematic name:
15-cis-4,4'-diapophytoene:FAD oxidoreductase (4,4'-diapolycopene-forming)
Reactions
-
31391 [IUBMB]15-cis-4,4'-diapophytoeneName origin: UniProt - CHECKED (C)Formula: C30H48
Charge: 0ChEBI compound status: CHECKED (C)4FADName origin: UniProt - CHECKED (C)Formula: C27H30N9O15P2
Charge: -3ChEBI compound status: CHECKED (C)4H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C)=all-trans-4,4'-diapolycopeneName origin: UniProt - CHECKED (C)Formula: C30H40
Charge: 0ChEBI compound status: CHECKED (C)4 -
15-cis-4,4'-diapophytoeneName origin: UniProt - CHECKED (C)Formula: C30H48
Charge: 0ChEBI compound status: CHECKED (C)3FADName origin: UniProt - CHECKED (C)Formula: C27H30N9O15P2
Charge: -3ChEBI compound status: CHECKED (C)3H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C)=all-trans-4,4'-diaponeurosporeneName origin: UniProt - CHECKED (C)Formula: C30H42
Charge: 0ChEBI compound status: CHECKED (C)3
Comments:
The enzyme catalyses four successive dehydrogenations, resulting in production of 4,4'-diapolycopene. While the enzyme from Staphylococcus aureus was only shown to produce 4,4'-diaponeurosporene in vivo [4], it is able to catalyse the last reaction in vitro [5].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
UniPathway
Gene Ontology:
GO:0102223
UniProtKB/Swiss-Prot:
CRTN_METSP
References
-
Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4'-diaponeurosporene of Staphylococcus aureus.J. Bacteriol. 176 : 7719-7726 (1994). [PMID: 8002598]
-
4,4'-diapophytoene desaturase: catalytic properties of an enzyme from the C30 carotenoid pathway of Staphylococcus aureus.J. Bacteriol. 181 : 6184-6187 (1999). [PMID: 10498735]
-
Functional properties of diapophytoene and related desaturases of C30 and C40 carotenoid biosynthetic pathways.Biochim. Biophys. Acta 1533 : 164-170 (2001). [PMID: 11566453]
-
Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde.Appl. Environ. Microbiol. 71 : 3294-3301 (2005). [PMID: 15933032]
-
Carotenoid production in Bacillus subtilis achieved by metabolic engineering.Biotechnol. Lett. 31 : 1789-1793 (2009). [PMID: 19618272]
[EC 1.3.8.2 created 2011, modified 2011]