IntEnz

EC 1.3.1.94 - Polyprenol reductase

IntEnz Enzyme Nomenclature
EC 1.3.1.94

Names

Reaction

• 34279 [IUBMB]
  di-trans,poly-cis-dolichol

  di-trans,poly-cis-dolichol

  POLYMER:9521

  Formula: C20H36O(C5H8)_n
  Charge: (0)(0)_n

  ChEBI polymer: CHEBI:16091

  

  +

  NADP⁺

  NADP(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:58349

  Formula: C21H25N7O17P3
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  =

  di-trans,cis-polyprenol

  di-trans,cis-polyprenol

  POLYMER:9525

  Formula: C20H34O(C5H8)_n
  Charge: (0)(0)_n

  ChEBI polymer: CHEBI:67132

  

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  NADPH

  NADPH

  Name origin: UniProt - CHECKED (C)

  CHEBI:57783

  Formula: C21H26N7O17P3
  Charge: -4

  ChEBI compound status: CHECKED (C)

  

Comments:

The reaction occurs in the reverse direction with reduction of the terminal double bond next to the alcohol group. Isolated from human fetal brain tissue but present in all eukaryotes. In mammalian cells dolichols are predominantly 18-21 isoprene units in length.

Links to other databases

References

1. Sagami, H., Kurisaki, A., Ogura, K.
   Formation of dolichol from dehydrodolichol is catalyzed by NADPH-dependent reductase localized in microsomes of rat liver.
   J. Biol. Chem. 268: 10109-10113 (1993). [PMID: 8486680]
2. Cantagrel, V., Lefeber, D. J., Ng, B. G., Guan, Z., Silhavy, J. L., Bielas, S. L., Lehle, L., Hombauer, H., Adamowicz, M., Swiezewska, E., De Brouwer, A. P., Blumel, P., Sykut-Cegielska, J., Houliston, S., Swistun, D., Ali, B. R., Dobyns, W. B., Babovic-Vuksanovic, D., van Bokhoven, H., Wevers, R. A., Raetz, C. R., Freeze, H. H., Morava, E., Al-Gazali, L., Gleeson, J. G.
   SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder.
   Cell 142: 203-217 (2010). [PMID: 20637498]

[EC 1.3.1.94 created 2012]