EC 1.23.1.1 - (+)-pinoresinol reductase

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IntEnz Enzyme Nomenclature
EC 1.23.1.1

Names

Accepted name:
(+)-pinoresinol reductase
Other names:
pinoresinol/lariciresinol reductase
pinoresinol-lariciresinol reductases
(+)-pinoresinol/(+)-lariciresinol
(+)-pinoresinol-(+)-lariciresinol reductase
PLR
Systematic name:
(+)-lariciresinol:NADP+ oxidoreductase

Reaction

Comments:

The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that further reduces the product to the lignan (−)-secoisolariciresinol [EC 1.23.1.2, (+)-lariciresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) [3], Linum perenne (perennial flax) [5] and Linum corymbulosum [6]. The 4-pro-R hydrogen of NADH is transferred to the 7-pro-R position of lariciresinol [1].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Chu, A., Dinkova, A., Davin, L. B., Bedgar, D. L., Lewis, N. G.
    Stereospecificity of (+)-pinoresinol and (+)-lariciresinol reductases from Forsythia intermedia.
    J. Biol. Chem. 268: 27026-27033 (1993). [PMID: 8262939]
  2. Dinkova-Kostova, A. T., Gang, D. R., Davin, L. B., Bedgar, D. L., Chu, A., Lewis, N. G.
    (+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia. Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase.
    J. Biol. Chem. 271: 29473-29482 (1996). [PMID: 8910615]
  3. Fujita, M., Gang, D. R., Davin, L. B., Lewis, N. G.
    Recombinant pinoresinol-lariciresinol reductases from western red cedar (Thuja plicata) catalyze opposite enantiospecific conversions.
    J. Biol. Chem. 274: 618-627 (1999). [PMID: 9872995]
  4. Min, T., Kasahara, H., Bedgar, D. L., Youn, B., Lawrence, P. K., Gang, D. R., Halls, S. C., Park, H., Hilsenbeck, J. L., Davin, L. B., Lewis, N. G., Kang, C.
    Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases.
    J. Biol. Chem. 278: 50714-50723 (2003). [PMID: 13129921]
  5. Hemmati, S., Schmidt, T. J., Fuss, E.
    (+)-Pinoresinol/(-)-lariciresinol reductase from Linum perenne Himmelszelt involved in the biosynthesis of justicidin B.
    FEBS Lett. 581: 603-610 (2007). [PMID: 17257599]
  6. Bayindir, U., Alfermann, A. W., Fuss, E.
    Hinokinin biosynthesis in Linum corymbulosum Reichenb.
    Plant J. 55: 810-820 (2008). [PMID: 18489708]

[EC 1.23.1.1 created 2013]