EC 1.2.7.7 - 3-methyl-2-oxobutanoate dehydrogenase (ferredoxin)

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IntEnz Enzyme Nomenclature
EC 1.2.7.7

Names

Accepted name:
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin)
Other names:
2-ketoisovalerate ferredoxin reductase
2-oxoisovalerate ferredoxin reductase
3-methyl-2-oxobutanoate synthase (ferredoxin)
VOR
branched-chain ketoacid ferredoxin reductase
branched-chain oxo acid ferredoxin reductase
keto-valine-ferredoxin oxidoreductase
ketoisovalerate ferredoxin reductase
ketoisovalerate oxidoreductase
Systematic name:
3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)

Reaction

Cofactors

Comments:

The enzyme is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, and 2-oxobutanoate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that reversibly catalyse the oxidative decarboxylation of different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase, and EC 1.2.7.3, 2-oxoglutarate synthase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0043807
UniProtKB/Swiss-Prot: (23) [show] [UniProt]

References

  1. Heider, J., Mai, X.H. and Adams, M.W.W.
    Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.
    J. Bacteriol. 178: 780-787 (1996). [PMID: 8550513]
  2. Tersteegen, A., Linder, D., Thauer, R.K. and Hedderich, R.
    Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum.
    Eur. J. Biochem. 244: 862-868 (1997). [PMID: 9108258]
  3. Schut, G.J., Menon, A.L. and Adams, M.W.W.
    2-Keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis.
    Methods Enzymol. 331: 144-158 (2001). [PMID: 11265457]
  4. Buchanan, B. B.
    Role of ferredoxin in the synthesis of alpha-ketobutyrate from propionyl coenzyme A and carbon dioxide by enzymes from photosynthetic and nonphotosynthetic bacteria.
    J. Biol. Chem. 244: 4218-4223 (1969). [PMID: 5800441]

[EC 1.2.7.7 created 2003]