EC 1.2.5.1 - Pyruvate dehydrogenase (quinone)

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IntEnz Enzyme Nomenclature
EC 1.2.5.1

Names

Accepted name:
pyruvate dehydrogenase (quinone)
Other names:
pyruvate dehydrogenase
pyruvic dehydrogenase
pyruvic (cytochrome b1) dehydrogenase
pyruvate:ubiquinone-8-oxidoreductase
pyruvate oxidase [ambiguous]
pyruvate dehydrogenase (cytochrome) [incorrect]
Systematic name:
pyruvate:ubiquinone oxidoreductase

Reaction

Cofactor

Comments:

Flavoprotein (FAD) [1]. This bacterial enzyme is located on the inner surface of the cytoplasmic membrane and coupled to the respiratory chain via ubiquinone [2,3]. Does not accept menaquinone. Activity is greatly enhanced by lipids [4,5,6]. Requires thiamine diphosphate [7]. The enzyme can also form acetoin [8].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052737
UniProtKB/Swiss-Prot:

References

  1. Recny, M. A., Hager, L. P.
    Reconstitution of native Escherichia coli pyruvate oxidase from apoenzyme monomers and FAD.
    J. Biol. Chem. 257: 12878-12886 (1982). [PMID: 6752142]
  2. Cunningham, C. C., Hager, L. P.
    Reactivation of the lipid-depleted pyruvate oxidase system from Escherichia coli with cell envelope neutral lipids.
    J. Biol. Chem. 250: 7139-7146 (1975). [PMID: 1100621]
  3. Koland, J. G., Miller, M. J., Gennis, R. B.
    Reconstitution of the membrane-bound, ubiquinone-dependent pyruvate oxidase respiratory chain of Escherichia coli with the cytochrome d terminal oxidase.
    Biochemistry 23: 445-453 (1984). [PMID: 6367818]
  4. Grabau, C., Cronan, J. E.
    In vivo function of Escherichia coli pyruvate oxidase specifically requires a functional lipid binding site.
    Biochemistry 25: 3748-3751 (1986). [PMID: 3527254]
  5. Wang, A. Y., Chang, Y. Y., Cronan, J. E.
    Role of the tetrameric structure of Escherichia coli pyruvate oxidase in enzyme activation and lipid binding.
    J. Biol. Chem. 266: 10959-10966 (1991). [PMID: 2040613]
  6. Chang, Y. Y., Cronan, J. E.
    Sulfhydryl chemistry detects three conformations of the lipid binding region of Escherichia coli pyruvate oxidase.
    Biochemistry 36: 11564-11573 (1997). [PMID: 9305946]
  7. O'Brien, T. A., Schrock, H. L., Russell, P., Blake, R., Gennis, R. B.
    Preparation of Escherichia coli pyruvate oxidase utilizing a thiamine pyrophosphate affinity column.
    Biochim. Biophys. Acta 452: 13-29 (1976). [PMID: 791368]
  8. Bertagnolli, B. L., Hager, L. P.
    Role of flavin in acetoin production by two bacterial pyruvate oxidases.
    Arch. Biochem. Biophys. 300: 364-371 (1993). [PMID: 8424670]

[EC 1.2.5.1 created 2010]