EC - Methylmalonate-semialdehyde dehydrogenase (CoA acylating)

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
methylmalonate-semialdehyde dehydrogenase (CoA acylating)
Other names:
MMSA dehydrogenase
iolA (gene name)
methylmalonate-semialdehyde dehydrogenase (acylating)
Systematic name:
2-methyl-3-oxopropanoate:NAD+ 3-oxidoreductase (CoA-propanoylating)



Also converts 3-oxopropanoate into acetyl-CoA [3]. The reaction occurs in two steps with the decarboxylation process preceding CoA-binding [3]. Bicarbonate rather than CO2 is released as a final product [3].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00068
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004491
CAS Registry Number: 37205-49-5
UniProtKB/Swiss-Prot: (54) [show] [UniProt]


  1. Sokatch, J.R., Sanders, L.E. and Marshall, V.P.
    Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine.
    J. Biol. Chem. 243 : 2500-2506 (1968). [PMID: 4297649]
  2. Dubourg, H., Stines-Chaumeil, C., Didierjean. C., Talfournier, F., Rahuel-Clermont, S., Branlant, G. and Aubry, A.
    Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis.
    Acta Crystallogr. D Biol. Crystallogr. 60 : 1435-1437 (2004). [PMID: 15272169]
  3. Stines-Chaumeil, C., Talfournier, F. and Branlant, G.
    Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis
    Biochem. J. 395 : 107-115 (2006). [PMID: 16332250]

[EC created 1972, modified 2014]