EC - Juglone 3-hydroxylase

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IntEnz Enzyme Nomenclature


Accepted name:
juglone 3-hydroxylase
Other names:
juglone hydroxylase
naphthoquinone hydroxylase
Systematic name:
5-hydroxy-1,4-naphthoquinone,water:oxygen oxidoreductase (3-hydroxylating)



Even though oxygen is consumed, molecular oxygen is not incorporated into the product. Catalysis starts by incorporation of an oxygen atom from a water molecule into the substrate. The naphthalene-1,2,4,8-tetrol intermediate is then oxidized by molecular oxygen, which is reduced to water. Also acts on 1,4-naphthoquinone, naphthazarin and 2-chloro-1,4-naphthoquinone. Formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ERGO , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
CAS Registry Number: 98865-54-4


  1. Rettenmaier, H. and Lingens, F.
    Purification and some properties of two isofunctional juglone hydroxylases from Pseudomonas putida J1.
    Biol. Chem. Hoppe-Seyler 366 : 637-646 (1985). [PMID: 4041238]

[EC created 1989 as EC, transferred 2016 to EC]