EC - 4-methoxybenzoate monooxygenase (O-demethylating)

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
4-methoxybenzoate monooxygenase (O-demethylating)
Other names:
4-methoxybenzoate 4-monooxygenase (O-demethylating)
4-methoxybenzoate O-demethylase
p-anisic O-demethylase
Systematic name:
4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating)




The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018690
CAS Registry Number: 37256-78-3


  1. Bernhardt, F.-H., Nastainczyk, W. and Seydewitz, V.
    Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida.
    Eur. J. Biochem. 72 : 107-115 (1977). [PMID: 188654]
  2. Paszcynski, A. and Trojanowski, J.
    An affinity-column procedure for the purification of veratrate O-demethylase from fungi.
    Microbios 18 : 111-121 (1977). [PMID: 25369]
  3. Twilfer, H., Bernhardt, F.-H. and Gersonde, K.
    An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida.
    Eur. J. Biochem. 119 : 595-602 (1981). [PMID: 6273164]

[EC created 1972]