EC - 3-[(E)-2-isocyanoethenyl]-1H-indole synthase

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IntEnz Enzyme Nomenclature


Accepted name:
3-[(E)-2-isocyanoethenyl]-1H-indole synthase
Other name:
isnB (gene name)
Systematic name:
(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate,2-oxoglutarate:oxygen oxidoreductase (decarboxylating, 3-[(E)-2-isocyanoethenyl]-1H-indole-forming)



The enzyme has been characterized from an unidentified soil bacterium. It catalyses an Fe2+, 2-oxoglutarate-dependent monooxygenation at C-3, which is followed by decarboxylation and dehydration, resulting in the generation of a trans C-C double bond. cf. EC, L-tryptophan isonitrile desaturase/decarboxylase (3-[(Z)-2-isocyanoethenyl]-1H-indole-forming).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Brady, S. F., Clardy, J.
    Cloning and heterologous expression of isocyanide biosynthetic genes from environmental DNA.
    Angew. Chem. Int. Ed. Engl. 44 : 7063-7065 (2005). [PMID: 16206308]
  2. Chang, W. C., Sanyal, D., Huang, J. L., Ittiamornkul, K., Zhu, Q., Liu, X.
    In Vitro Stepwise Reconstitution of Amino Acid Derived Vinyl Isocyanide Biosynthesis: Detection of an Elusive Intermediate.
    Org. Lett. 19 : 1208-1211 (2017). [PMID: 28212039]

[EC created 2018]