EC - CMP-N-acetylneuraminate monooxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
CMP-N-acetylneuraminate monooxygenase
Other names:
N-acetylneuraminic monooxygenase
CMP-N-acetylneuraminic acid hydroxylase
CMP-Neu5Ac hydroxylase
cytidine monophosphoacetylneuraminate monooxygenase
cytidine-5'-monophosphate-N-acetylneuraminic acid hydroxylase
Systematic name:
CMP-N-acetylneuraminate,ferrocytochrome-b5:oxygen oxidoreductase (N-acetyl-hydroxylating)




This enzyme contains both a Rieske-type [2Fe-2S] cluster and a second iron site. The ferricytochrome b5 produced is reduced by NADH and cytochrome-b5 reductase (EC The enzyme can be activated by Fe2+ or Fe3+.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0030338
CAS Registry Number: 116036-67-0
UniProtKB/Swiss-Prot: (11) [show] [UniProt]


  1. Shaw, L. and Schauer, R.
    The biosynthesis of N-glycoloylneuraminic acid occurs by hydroxylation of the CMP-glycoside of N-acetylneuraminic acid.
    Biol. Chem. Hoppe-Seyler 369: 477-486 (1988). [PMID: 3202954]
  2. Kozutsumi, Y., Kawano, T., Yamakawa, T. and Suzuki, A.
    Participation of cytochrome b5 in CMP-N-acetylneuraminic acid hydroxylation in mouse liver cytosol.
    J. Biochem. (Tokyo) 109: 704-706 (1990). [PMID: 1964451]
  3. Schneckenburger, P., Shaw, L. and Schauer, R.
    Purification, characterization and reconstitution of CMP-N-acetylneuraminate hydroxylase from mouse liver.
    Glycoconj. J. 11: 194-203 (1994). [PMID: 7841794]
  4. Kawano, T., Koyama, S., Takematsu, H., Kozutsumi, Y., Kawasaki, H., Kawashima, S., Kawasaki, T. and Suzuki, A.
    Molecular cloning of cytidine monophospho-N-acetylneuraminic acid hydroxylase. Regulation of species- and tissue-specific expression of N-glycolylneuraminic acid.
    J. Biol. Chem. 270: 16458-16463 (1995). [PMID: 7608218]
  5. Schlenzka, W., Shaw, L., Kelm, S., Schmidt, C.L., Bill, E., Trautwein, A.X., Lottspeich, F. and Schauer, R.
    CMP-N-acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron-sulphur protein to be described in Eukarya.
    FEBS Lett. 385: 197-200 (1996). [PMID: 8647250]

[EC created 1992 as EC, transferred 2003 to EC]