EC 1.14.15.8 - Steroid 15β-monooxygenase

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IntEnz Enzyme Nomenclature
EC 1.14.15.8

Names

Accepted name:
steroid 15β-monooxygenase
Other names:
cytochrome P-450meg
cytochrome P450meg
steroid 15β-hydroxylase
CYP106A2
BmCYP106A2
Systematic name:
progesterone,reduced-ferredoxin:oxygen oxidoreductase (15β-hydroxylating)

Reaction

Comments:

The enzyme from Bacillus megaterium hydroxylates a variety of 3-oxo-Δ4-steroids in position 15β. Ring A-reduced, aromatic, and 3β-hydroxy-Δ4-steroids do not serve as substrates [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00081
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Berg, A., Ingelman-Sundberg, M., Gustafsson, J. A.
    Purification and characterization of cytochrome P-450meg.
    J. Biol. Chem. 254 : 5264-5271 (1979). [PMID: 109432]
  2. Berg, A., Gustafsson, J. A., Ingelman-Sundberg, M.
    Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium.
    J. Biol. Chem. 251 : 2831-2838 (1976). [PMID: 177422]
  3. Lisurek, M., Kang, M. J., Hartmann, R. W., Bernhardt, R.
    Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry.
    Biochem. Biophys. Res. Commun. 319 : 677-682 (2004). [PMID: 15178459]
  4. Goni, G., Zollner, A., Lisurek, M., Velazquez-Campoy, A., Pinto, S., Gomez-Moreno, C., Hannemann, F., Bernhardt, R., Medina, M.
    Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.
    Biochim. Biophys. Acta 1794 : 1635-1642 (2009). [PMID: 19635596]
  5. Lisurek, M., Simgen, B., Antes, I., Bernhardt, R.
    Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation.
    Chembiochem 9 : 1439-1449 (2008). [PMID: 18481342]

[EC 1.14.15.8 created 2010]