EC 1 - Oxidoreductases
EC 1.14 - Acting on paired donors, with incorporation or reduction of molecular oxygen. The oxygen incorporated need not be derived from O2
EC 1.14.14 - With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
EC 1.14.14.9 - 4-hydroxyphenylacetate 3-monooxygenase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.14.14.9
Names
Accepted name:
4-hydroxyphenylacetate 3-monooxygenase
Other
names:
4 HPA 3-hydroxylase
4-hydroxyphenylacetic acid-3-hydroxylase
p-hydroxyphenylacetate 3-hydroxylase
p-hydroxyphenylacetate hydroxylase
p-hydroxyphenylacetate hydroxylase (FAD)
p-hydroxyphenylacetate 3-hydroxylase (FAD)
HpaB
4-hydroxyphenylacetic acid-3-hydroxylase
p-hydroxyphenylacetate 3-hydroxylase
p-hydroxyphenylacetate hydroxylase
p-hydroxyphenylacetate hydroxylase (FAD)
p-hydroxyphenylacetate 3-hydroxylase (FAD)
HpaB
Systematic name:
4-hydroxyphenylacetate,FAD:oxygen oxidoreductase (3-hydroxylating)
Reaction
- 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O
Cofactor
Comments:
The enzyme from Escherichia coli attacks a broad spectrum of phenolic compounds. The enzyme uses FADH2 as a substrate rather than a cofactor [4]. FADH2 is provided by EC 1.5.1.36, flavin reductase (NADH) [5,6].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
UM-BBD
,
UniPathway
Gene Ontology:
GO:0052881
,
GO:0018660
CAS Registry Number:
37256-71-6
UniProtKB/Swiss-Prot:
HPAB_ECOLX
HPAB_KLEOX
HPAB_PSEAE
HPAB_THET8
HPAH_ACIBA
HPAH_GEOP9
HPAH_GEOTN
YOAI_BACSU
References
-
Metabolism of p-hydroxyphenylacetic acid in Pseudomonas ovalis.Biochim. Biophys. Acta 93 : 483-493 (1964).
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Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range.J. Bacteriol. 175 : 2162-2167 (1993). [PMID: 8458860]
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Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of Escherichia coli. A two-protein component enzyme.J. Biol. Chem. 269 : 22823-22829 (1994). [PMID: 8077235]
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Characterization of 4-hydroxyphenylacetate 3-hydroxylase (HpaB) of Escherichia coli as a reduced flavin adenine dinucleotide-utilizing monooxygenase.Appl. Environ. Microbiol. 66 : 481-486 (2000). [PMID: 10653707]
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Functional analysis of the small component of the 4-hydroxyphenylacetate 3-monooxygenase of Escherichia coli W: a prototype of a new flavin:NAD(P)H reductase subfamily.J. Bacteriol. 182 : 627-636 (2000). [PMID: 10633095]
-
Coordinated production and utilization of FADH2 by NAD(P)H-flavin oxidoreductase and 4-hydroxyphenylacetate 3-monooxygenase.Biochemistry 42 : 7509-7517 (2003). [PMID: 12809507]
[EC 1.14.14.9 created 1972 as EC 1.14.13.3, transferred 2011 to EC 1.14.14.9]