EC - Flavonoid 3'-monooxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
flavonoid 3'-monooxygenase
Other names:
NADPH:flavonoid-3'-hydroxylase [incorrect]
flavonoid 3'-hydroxylase
flavonoid 3-hydroxylase [incorrect]
flavonoid 3-monooxygenase [incorrect]
Systematic name:
flavonoid,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)



A cytochrome P450 (heme-thiolate) protein found in plants. Acts on a number of flavonoids, including the flavanone naringenin and the flavone apigenin. Does not act on 4-coumarate or 4-coumaroyl-CoA. Formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
CAS Registry Numbers: 75991-44-5 85340-98-3


  1. Forkmann, G., Heller, W. and Grisebach, H.
    Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
    Z. Naturforsch. C: Biosci. 35: 691-695 (1980).
  2. Brugliera, F., Barri-Rewell, G., Holton, T. A., Mason, J. G.
    Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
    Plant J. 19: 441-451 (1999). [PMID: 10504566]
  3. Schoenbohm, C., Martens, S., Eder, C., Forkmann, G., Weisshaar, B.
    Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.
    Biol. Chem. 381: 749-753 (2000). [PMID: 11030432]

[EC created 1983 as EC, transferred 2018 to EC]