EC - Dibenzothiophene sulfone monooxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
dibenzothiophene sulfone monooxygenase
Other name:
dszA (gene name)
Systematic name:
dibenzothiophene-5,5-dioxide,FMNH2:oxygen oxidoreductase



This bacterial enzyme catalyses a step in the desulfurization pathway of dibenzothiophenes. The enzyme forms a two-component system with a dedicated NADH-dependent FMN reductase (EC encoded by the dszD gene, which also interacts with EC, dibenzothiophene monooxygenase. The flavin-N5-oxide that is formed by the enzyme reacts spontaneously with NADH to give oxidized flavin, releasing a water molecule.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Gray, K. A., Pogrebinsky, O. S., Mrachko, G. T., Xi, L., Monticello, D. J., Squires, C. H.
    Molecular mechanisms of biocatalytic desulfurization of fossil fuels.
    Nat. Biotechnol. 14 : 1705-1709 (1996). [PMID: 9634856]
  2. Ohshiro, T., Kojima, T., Torii, K., Kawasoe, H., Izumi, Y.
    Purification and characterization of dibenzothiophene (DBT) sulfone monooxygenase, an enzyme involved in DBT desulfurization, from Rhodococcus erythropolis D-1.
    J. Biosci. Bioeng. 88 : 610-616 (1999). [PMID: 16232672]
  3. Konishi, J., Ishii, Y., Onaka, T., Ohta, Y., Suzuki, M., Maruhashi, K.
    Purification and characterization of dibenzothiophene sulfone monooxygenase and FMN-dependent NADH oxidoreductase from the thermophilic bacterium Paenibacillus sp. strain A11-2.
    J. Biosci. Bioeng. 90 : 607-613 (2000). [PMID: 16232919]
  4. Ohshiro, T., Ishii, Y., Matsubara, T., Ueda, K., Izumi, Y., Kino, K., Kirimura, K.
    Dibenzothiophene desulfurizing enzymes from moderately thermophilic bacterium Bacillus subtilis WU-S2B: purification, characterization and overexpression.
    J. Biosci. Bioeng. 100 : 266-273 (2005). [PMID: 16243275]
  5. Adak, S., Begley, T. P.
    Dibenzothiophene Catabolism Proceeds via a Flavin-N5-oxide Intermediate.
    J. Am. Chem. Soc. 138 : 6424-6426 (2016). [PMID: 27120486]
  6. Adak, S., Begley, T. P.
    Flavin-N5-oxide: A new, catalytic motif in flavoenzymology.
    Arch. Biochem. Biophys. 632 : 4-10 (2017). [PMID: 28784589]

[EC created 2016]