EC 1.14.14.145 - Abieta-7,13-dien-18-ol hydroxylase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature
EC 1.14.14.145

Names

Accepted name:
abieta-7,13-dien-18-ol hydroxylase
Other names:
CYP720B1
PtAO
abietadienol hydroxylase [ambiguous]
Systematic name:
abieta-7,13-dien-18-ol,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (18-hydroxylating)

Reaction

Cofactor

Comments:

A cytochrome P450 (heme-thiolate) protein. This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine) [1], and the gene encoding the enzyme has been identified in Pinus taeda (loblolly pine) [3]. The recombinant enzyme catalyses the oxidation of multiple diterpene alcohol and aldehydes, including levopimaradienol, isopimara-7,15-dienol, isopimara-7,15-dienal, dehydroabietadienol and dehydroabietadienal. It is not able to oxidize abietadiene.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00081
Structural data: CSA , EC2PDB
Gene Ontology: GO:0036204
UniProtKB/Swiss-Prot:

References

  1. Funk, C. and Croteau, R.
    Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis.
    Arch. Biochem. Biophys. 308 : 258-266 (1994). [PMID: 8311462]
  2. Funk, C., Lewinsohn, E., Vogel, B.S., Steele, C.L. and Croteau, R.
    Regulation of oleoresinosis in grand fir (Abies grandis) (Coordinate induction of monoterpene and diterpene cyclases and two cytochrome P450-dependent diterpenoid hydroxylases by stem wounding).
    Plant Physiol. 106 : 999-1005 (1994). [PMID: 12232380]
  3. Ro, D.-K., Arimura, G.-I., Lau, S.Y.W., Piers, E. and Bohlmann, J.
    Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase.
    Proc. Natl. Acad. Sci. USA 102 : 8060-8065 (2005). [PMID: 15911762]

[EC 1.14.14.145 created 2009 as EC 1.14.13.109, modified 2012, transferred 2018 to EC 1.14.14.145]