EC - (+)-menthofuran synthase

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IntEnz Enzyme Nomenclature


Accepted name:
(+)-menthofuran synthase
Other names:
menthofuran synthase
(+)-pulegone 9-hydroxylase
cytochrome P450 menthofuran synthase
Systematic name:
(+)-pulegone,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (9-hydroxylating)




A cytochrome P450 (heme-thiolate) protein. The conversion of substrate into product involves the hydroxylation of the syn-methyl (C9), intramolecular cyclization to the hemiketal and dehydration to the furan [1]. This is the second cytochrome P450-mediated step of monoterpene metabolism in peppermint, with the other step being catalysed by EC, (S)-limonene 3-monooxygenase [1].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00081
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052582


  1. Bertea, C.M., Schalk, M., Karp, F., Maffei, M. and Croteau, R.
    Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene.
    Arch. Biochem. Biophys. 390 : 279-286 (2001). [PMID: 11396930]
  2. Mahmoud, S.S. and Croteau, R.B.
    Menthofuran regulates essential oil biosynthesis in peppermint by controlling a downstream monoterpene reductase.
    Proc. Natl. Acad. Sci. USA 100 : 14481-14486 (2003). [PMID: 14623962]

[EC created 2008 as EC, transferred 2018 to EC]