EC - β-amyrin 28-monooxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
β-amyrin 28-monooxygenase
Other names:
β-amyrin 28-oxidase
Systematic name:
β-amyrin,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (28-hydroxylating)



A cytochrome P450 (heme-thiolate) protein found in plants. The enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro. The enzyme from Medicago truncatula (barrel medic) (CYP716A12) can also convert α-amyrin and lupeol to ursolic acid and betulinic acid, respectively. The enzyme from Maesa lanceolata (false assegai) (CYP16A75) does not catalyse the reaction to completion, resulting in accumulation of both intermediates. Formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Fukushima, E. O., Seki, H., Ohyama, K., Ono, E., Umemoto, N., Mizutani, M., Saito, K., Muranaka, T.
    CYP716A subfamily members are multifunctional oxidases in triterpenoid biosynthesis.
    Plant Cell Physiol. 52 : 2050-2061 (2011). [PMID: 22039103]
  2. Han, J. Y., Kim, M. J., Ban, Y. W., Hwang, H. S., Choi, Y. E.
    The involvement of ?-amyrin 28-oxidase (CYP716A52v2) in oleanane-type ginsenoside biosynthesis in Panax ginseng.
    Plant Cell Physiol. 54 : 2034-2046 (2013). [PMID: 24092881]
  3. Moses, T., Pollier, J., Faizal, A., Apers, S., Pieters, L., Thevelein, J. M., Geelen, D., Goossens, A.
    Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata.
    Mol Plant 8 : 122-135 (2015). [PMID: 25578277]

[EC created 2015 as EC, transferred 2018 to EC]