EC 1 - Oxidoreductases
EC 1.14 - Acting on paired donors, with incorporation or reduction of molecular oxygen. The oxygen incorporated need not be derived from O2
EC 1.14.14 - With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
EC 1.14.14.12 - 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.14.14.12
Names
Accepted name:
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Other
name:
HsaA
Systematic name:
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FADH2:oxygen oxidoreductase
Reaction
- 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O
Comments:
This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. It can use either FADH or FMNH2 as flavin cofactor. The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.
Links to other databases
Gene Ontology:
GO:0036383
UniProtKB/Swiss-Prot:
References
-
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.J. Biol. Chem. 285 : 22264-22275 (2010). [PMID: 20448045]
[EC 1.14.14.12 created 2011]