EC - 2,5-diketocamphane 1,2-monooxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
2,5-diketocamphane 1,2-monooxygenase
Other names:
2,5-diketocamphane lactonizing enzyme
2,5-diketocamphane 1,2-monooxygenase oxygenating component
ketolactonase I [ambiguous]
camP (gene name)
camphor 1,2-monooxygenase
camphor ketolactonase I
Systematic name:
(+)-bornane-2,5-dione,FMNH2:oxygen oxidoreductase (1,2-lactonizing)




A Baeyer-Villiger monooxygenase isolated from camphor-grown strains of Pseudomonas putida and encoded on the cam plasmid. Involved in the degradation of (+)-camphor. Requires a dedicated NADH-FMN reductase [cf. EC, FMN reductase (NADH)] [1-3]. Can accept several bicyclic ketones including (+)- and (−)-camphor [6] and adamantanone [4]. The product spontaneously converts to [(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetate.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ERGO , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB


  1. Conrad, H.E., DuBus, R., Namtvedt, M.J. and Gunsalus, I.C.
    Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton.
    J. Biol. Chem. 240 : 495-503 (1965). [PMID: 14253460]
  2. Yu, C.A. and Gunsalus, I.C.
    Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components.
    J. Biol. Chem. 244 : 6149-6152 (1969). [PMID: 4310834]
  3. Taylor, D. G., Trudgill, P. W.
    Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453.
    J. Bacteriol. 165 : 489-497 (1986). [PMID: 3944058]
  4. Selifonov, S. A.
    Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid.
    Biochem. Biophys. Res. Commun. 186 : 1429-1436 (1992). [PMID: 1510672]
  5. Jones, K. H., Smith, R. T., Trudgill, P. W.
    Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453.
    J. Gen. Microbiol. 139 : 797-805 (1993). [PMID: 8515237]
  6. Kadow, M., Sass, S., Schmidt, M., Bornscheuer, U. T.
    Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007.
    AMB Express 1 : 13 (2011). [PMID: 21906366]
  7. Iwaki, H., Grosse, S., Bergeron, H., Leisch, H., Morley, K., Hasegawa, Y., Lau, P. C.
    Camphor pathway redux: functional recombinant expression of 2,5- and 3,6-diketocamphane monooxygenases of Pseudomonas putida ATCC 17453 with their cognate flavin reductase catalyzing Baeyer-Villiger reactions.
    Appl. Environ. Microbiol. 79 : 3282-3293 (2013). [PMID: 23524667]

[EC created 1972 as EC, transferred 2012 to EC, transferred 2018 to EC]