EC 1.14.13.50 - Pentachlorophenol monooxygenase

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IntEnz Enzyme Nomenclature
EC 1.14.13.50

Names

Accepted name:
pentachlorophenol monooxygenase
Other names:
PCP hydroxylase
pentachlorophenol 4-monooxygenase
pentachlorophenol dechlorinase
pentachlorophenol dehalogenase
pentachlorophenol hydroxylase
PcpB
PCB 4-monooxygenase
PCB4MO
pcpB (gene name)
Systematic name:
pentachlorophenol,NADPH:oxygen oxidoreductase (hydroxylating, dechlorinating)

Reactions

Cofactor

Comments:

A flavoprotein (FAD). The enzyme displaces a diverse range of substituents from the 4-position of polyhalogenated phenols but requires that a halogen substituent be present at the 2-position [2]. If C-4 carries a halogen substituent, reaction 1 is catalysed; if C-4 is unsubstituted, reaction 2 is catalysed.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018677
CAS Registry Numbers: 124148-88-5 136111-57-4
UniProtKB/Swiss-Prot:

References

  1. Schenk, T., Müller, R., Mörsberger, F., Otto, M.K. and Lingens, F.
    Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790.
    J. Bacteriol. 171: 5487-5491 (1989). [PMID: 2793827]
  2. Xun, L., Topp, E. and Orser, C.S.
    Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries.
    J. Bacteriol. 174: 2898-2902 (1992). [PMID: 1569020]
  3. Xun, L., Topp, E. and Orser, C.S.
    Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase.
    J. Bacteriol. 174: 5745-5747 (1992). [PMID: 1512208]
  4. Lange, C.C., Schneider, B.J. and Orser, C.S.
    Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723.
    Biochem. Biophys. Res. Commun. 219: 146-149 (1996). [PMID: 8619798]
  5. Nakamura, T., Motoyama, T., Hirono, S. and Yamaguchi, I.
    Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase.
    Biochim. Biophys. Acta 1700: 151-159 (2004). [PMID: 15262224]
  6. Chen, L., Yang, J.
    Biochemical characterization of the tetrachlorobenzoquinone reductase involved in the biodegradation of pentachlorophenol.
    Int J Mol Sci 9: 198-212 (2008). [PMID: 19325743]
  7. Hlouchova, K., Rudolph, J., Pietari, J. M., Behlen, L. S., Copley, S. D.
    Pentachlorophenol hydroxylase, a poorly functioning enzyme required for degradation of pentachlorophenol by Sphingobium chlorophenolicum.
    Biochemistry 51: 3848-3860 (2012). [PMID: 22482720]
  8. Rudolph, J., Erbse, A. H., Behlen, L. S., Copley, S. D.
    A radical intermediate in the conversion of pentachlorophenol to tetrachlorohydroquinone by Sphingobium chlorophenolicum.
    Biochemistry 53: 6539-6549 (2014). [PMID: 25238136]

[EC 1.14.13.50 created 1992, modified 2005, modified 2017]