EC - Methane monooxygenase (soluble)

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IntEnz Enzyme Nomenclature


Accepted name:
methane monooxygenase (soluble)
Other name:
methane hydroxylase
Systematic name:
methane,NAD(P)H:oxygen oxidoreductase (hydroxylating)



The enzyme is soluble, in contrast to the particulate enzyme, EC Broad specificity; many alkanes can be hydroxylated, and alkenes are converted into the corresponding epoxides; CO is oxidized to CO2, ammonia is oxidized to hydroxylamine, and some aromatic compounds and cyclic alkanes can also be hydroxylated, but more slowly.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ERGO , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0015049
CAS Registry Number: 51961-97-8


  1. Colby, J. Stirling, D.I. and Dalton, H.
    The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.
    Biochem. J. 165: 395-402 (1977). [PMID: 411486]
  2. Hyman, M.R. and Wood, P.M.
    Methane oxidation by Nitrosomonas europaea.
    Biochem. J. 212: 31-37 (1983). [PMID: 6870854]
  3. Stirling, D.I. and Dalton, H.
    Properties of the methane mono-oxygenase from extracts of Methylosinus trichosporium OB3b and evidence for its similarity to the enzyme from Methylococcus capsulatus (Bath).
    Eur. J. Biochem. 96: 205-212 (1979). [PMID: 572296]
  4. Tonge, G.M., Harrison, D.E.F. and Higgins, I.J.
    Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b.
    Biochem. J. 161: 333-344 (1977). [PMID: 15544]

[EC created 1984, modified 2011]