EC - Butane monooxygenase (soluble)

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IntEnz Enzyme Nomenclature


Accepted name:
butane monooxygenase (soluble)
Other names:
bmoBCDXYZ (gene names)
Systematic name:
butane,NADH:oxygen oxidoreductase




The enzyme, characterized from the bacterium Thauera butanivorans, is similar to EC, methane monooxygenase (soluble), but has a very low activity with methane. It comprises three components - a carboxylate-bridged non-heme di-iron center-containing hydroxylase (made of three different subunits), a flavo- iron sulfur-containing NADH-oxidoreductase, and a small regulatory component protein. The enzyme can also act on other C3-C6 linear and branched aliphatic alkanes with lower activity.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Sluis, M. K., Sayavedra-Soto, L. A., Arp, D. J.
    Molecular analysis of the soluble butane monooxygenase from 'Pseudomonas butanovora'.
    Microbiology (Reading, Engl.) 148 : 3617-3629 (2002). [PMID: 12427952]
  2. Dubbels, B. L., Sayavedra-Soto, L. A., Arp, D. J.
    Butane monooxygenase of 'Pseudomonas butanovora': purification and biochemical characterization of a terminal-alkane hydroxylating diiron monooxygenase.
    Microbiology (Reading, Engl.) 153 : 1808-1816 (2007). [PMID: 17526838]
  3. Doughty, D. M., Kurth, E. G., Sayavedra-Soto, L. A., Arp, D. J., Bottomley, P. J.
    Evidence for involvement of copper ions and redox state in regulation of butane monooxygenase in Pseudomonas butanovora.
    J. Bacteriol. 190 : 2933-2938 (2008). [PMID: 18281403]
  4. Cooley, R. B., Dubbels, B. L., Sayavedra-Soto, L. A., Bottomley, P. J., Arp, D. J.
    Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly 'Pseudomonas butanovora'.
    Microbiology (Reading, Engl.) 155 : 2086-2096 (2009). [PMID: 19383682]

[EC created 2016]