IntEnz

EC 1.14.13.215 - Protoasukamycin 4-monooxygenase

IntEnz Enzyme Nomenclature
EC 1.14.13.215

Names

Reaction

• 49044 [IUBMB]
  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  NADH

  NADH

  Name origin: UniProt - CHECKED (C)

  CHEBI:57945

  Formula: C21H27N7O14P2
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  O₂

  O2

  Name origin: UniProt - CHECKED (C)

  CHEBI:15379

  Formula: O2
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  protoasukamycin

  protoasukamycin

  Name origin: UniProt - CHECKED (C)

  CHEBI:90901

  Formula: C31H34N2O5
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  =

  4-hydroxyprotoasukamycin

  4-hydroxyprotoasukamycin

  Name origin: UniProt - CHECKED (C)

  CHEBI:90902

  Formula: C31H34N2O6
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  H₂O

  H2O

  Name origin: UniProt - CHECKED (C)

  CHEBI:15377

  Formula: H2O
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  NAD⁺

  NAD(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:57540

  Formula: C21H26N7O14P2
  Charge: -1

  ChEBI compound status: CHECKED (C)

  

Cofactor

• flavin

Comments:

The enzyme, characterized from the bacterium Streptomyces nodosus subsp. asukaensis, is involved in the biosynthesis of the antibiotic asukamycin. Requires a flavin cofactor, with no preference among FMN, FAD or riboflavin. When flavin concentration is low, activity is enhanced by the presence of the NADH-dependent flavin-reductase AsuE2.

Links to other databases

References

1. Rui, Z., Sandy, M., Jung, B., Zhang, W.
   Tandem enzymatic oxygenations in biosynthesis of epoxyquinone pharmacophore of manumycin-type metabolites.
   Chem. Biol. 20 : 879-887 (2013). [PMID: 23890006]

[EC 1.14.13.215 created 2016]