EC - 1,3,7-trimethyluric acid 5-monooxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
1,3,7-trimethyluric acid 5-monooxygenase
Other name:
tmuM (gene name)
Systematic name:
1,3,7-trimethylurate,NADH:oxygen oxidoreductase (1,3,7-trimethyl-5-hydroxyisourate forming)



The enzyme, characterized from the bacterium Pseudomonas sp. CBB1, is part of the bacterial C-8 oxidation-based caffeine degradation pathway. The product decomposes spontaneously to a racemic mixture of 3,6,8-trimethylallantoin. The enzyme shows no acitivity with urate. cf. EC, FAD-dependent urate hydroxylase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Mohanty, S. K., Yu, C. L., Das, S., Louie, T. M., Gakhar, L., Subramanian, M.
    Delineation of the caffeine C-8 oxidation pathway in Pseudomonas sp. strain CBB1 via characterization of a new trimethyluric acid monooxygenase and genes involved in trimethyluric acid metabolism.
    J. Bacteriol. 194 : 3872-3882 (2012). [PMID: 22609920]
  2. Summers, R. M., Mohanty, S. K., Gopishetty, S., Subramanian, M.
    Genetic characterization of caffeine degradation by bacteria and its potential applications.
    Microb Biotechnol 8 : 369-378 (2015). [PMID: 25678373]

[EC created 2016]