EC 1.14.13.181 - 13-deoxydaunorubicin hydroxylase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature
EC 1.14.13.181

Names

Accepted name:
13-deoxydaunorubicin hydroxylase
Other name:
DoxA
Systematic name:
13-deoxydaunorubicin,NADPH:oxygen oxidoreductase (13-hydroxylating)

Reactions

Comments:

The enzymes from the Gram-positive bacteria Streptomyces sp. C5 and Streptomyces peucetius show broad substrate specificity for structures based on an anthracycline aglycone, but have a strong preference for 4-methoxy anthracycline intermediates (13-deoxydaunorubicin and 13-dihydrodaunorubicin) over their 4-hydroxy analogues (13-deoxycarminomycin and 13-dihydrocarminomycin), as well as a preference for substrates hydroxylated at the C-13 rather than the C-14 position.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Walczak, R. J., Dickens, M. L., Priestley, N. D., Strohl, W. R.
    Purification, properties, and characterization of recombinant Streptomyces sp. strain C5 DoxA, a cytochrome P-450 catalyzing multiple steps in doxorubicin biosynthesis.
    J. Bacteriol. 181: 298-304 (1999). [PMID: 9864343]
  2. Dickens, M. L., Priestley, N. D., Strohl, W. R.
    In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA.
    J. Bacteriol. 179: 2641-2650 (1997). [PMID: 9098063]

[EC 1.14.13.181 created 2013]