EC 1.14.12.24 - 2,4-dinitrotoluene dioxygenase

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IntEnz Enzyme Nomenclature
EC 1.14.12.24

Names

Accepted name:
2,4-dinitrotoluene dioxygenase
Other name:
dntA (gene name)
Systematic name:
2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating,nitrite-releasing)

Reaction

Comments:

This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3 ferredoxin—NAD+ reductase), and an α3β3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23 nitroarene dioxygenase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Suen, W. C., Haigler, B. E., Spain, J. C.
    2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase.
    J. Bacteriol. 178 : 4926-4934 (1996). [PMID: 8759857]

[EC 1.14.12.24 created 2015]