EC - Toluene dioxygenase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
toluene dioxygenase
Other name:
toluene 2,3-dioxygenase
Systematic name:
toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)



A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis-dihydrodiols.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018624
CAS Registry Number: 120038-36-0


  1. Renganathan, V.
    Possible involvement of toluene-2,3-dioxygenase in defluorination of 3-fluoro-substituted benzenes by toluene-degrading Pseudomonas sp. strain T-12.
    Appl. Exp. Microbiol. 55 : 330-334 (1989).
  2. Subramanian, V., Liu, T.-N., Yeh, W.K. and Gibson, D.T.
    Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography.
    Biochem. Biophys. Res. Commun. 91 : 1131-1139 (1979). [PMID: 526270]

[EC created 1992]