EC - Procollagen-proline 3-dioxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
procollagen-proline 3-dioxygenase
Other names:
proline,2-oxoglutarate 3-dioxygenase
prolyl 3-hydroxylase
prolyl-4-hydroxyprolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase, 3-hydroxylating
protocollagen proline 3-hydroxylase
procollagen-proline,2-oxoglutarate 3-dioxygenase
Systematic name:
[procollagen]-L-proline,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)




Requires Fe2+ and ascorbate. The enzyme forms a complex with protein disulfide isomerase, and is located in the endoplasmic reticulum. It modifies proline residues within the procollagen peptide of certain collagen types. The modification is essential for proper collagen triple helix formation.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC51471
Structural data: CSA , EC2PDB
Gene Ontology: GO:0019797
CAS Registry Number: 63551-75-7
UniProtKB/Swiss-Prot: (17) [show] [UniProt]


  1. Risteli, J., Tryggvason, K. and Kivirikko, K.I.
    Prolyl 3-hydroxylase: partial characterization of the enzyme from rat kidney cortex.
    Eur. J. Biochem. 73 : 485-492 (1977). [PMID: 191255]
  2. Risteli, J., Tryggvason, K. and Kivirikko, K.I.
    A rapid assay for prolyl 3-hydroxylase activity.
    Anal. Biochem. 84 : 423-431 (1978). [PMID: 204218]
  3. Vranka, J. A., Sakai, L. Y., Bachinger, H. P.
    Prolyl 3-hydroxylase 1, enzyme characterization and identification of a novel family of enzymes.
    J. Biol. Chem. 279 : 23615-23621 (2004). [PMID: 15044469]
  4. Tiainen, P., Pasanen, A., Sormunen, R., Myllyharju, J.
    Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV.
    J. Biol. Chem. 283 : 19432-19439 (2008). [PMID: 18487197]

[EC created 1981, modified 1983, modified 2017]