EC 1 - Oxidoreductases
EC 1.14 - Acting on paired donors, with incorporation or reduction of molecular oxygen. The oxygen incorporated need not be derived from O2
EC 1.14.11 - With 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
EC 1.14.11.39 - L-asparagine oxygenase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.14.11.39
Names
Accepted name:
L-asparagine oxygenase
Other
names:
L-asparagine 3-hydroxylase
AsnO
AsnO
Systematic name:
L-asparagine,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Reaction
- L-asparagine + 2-oxoglutarate + O2 = (2S,3S)-3-hydroxyasparagine + succinate + CO2
Cofactor
Comments:
Requires Fe2+. The enzyme is only able to hydroxylate free L-asparagine. It is not active toward D-asparagine. The β-hydroxylated asparagine produced is incorporated at position 9 of the calcium-dependent antibiotic (CDA), an 11-residue non-ribosomally synthesized acidic lipopeptide lactone.
Links to other databases
UniProtKB/Swiss-Prot:
ASNO_STRCO
References
-
Mechanistic and structural basis of stereospecific Cβ-hydroxylation in calcium-dependent antibiotic, a daptomycin-type lipopeptide.ACS Chem. Biol. 2 : 187-196 (2007). [PMID: 17373765]
[EC 1.14.11.39 created 2013]