EC 1.14.11.33 - DNA oxidative demethylase

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IntEnz Enzyme Nomenclature
EC 1.14.11.33

Names

Accepted name:
DNA oxidative demethylase
Other names:
alkylated DNA repair protein
α-ketoglutarate-dependent dioxygenase ABH1
alkB (gene name)
Systematic name:
methyl DNA-base, 2-oxoglutarate:oxygen oxidoreductase (formaldehyde-forming)

Reaction

Cofactor

Comments:

Contains iron; activity is slightly stimulated by ascorbate. Catalyses oxidative demethylation of the DNA base lesions N1-methyladenine, N3-methylcytosine, N1-methylguanine, and N3-methylthymine. It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0103053
UniProtKB/Swiss-Prot: (10) [show] [UniProt]

References

  1. Falnes, P. O., Johansen, R. F., Seeberg, E.
    AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli.
    Nature 419: 178-182 (2002). [PMID: 12226668]
  2. Yi, C., Yang, C. G., He, C.
    A non-heme iron-mediated chemical demethylation in DNA and RNA.
    Acc. Chem. Res. 42: 519-529 (2009). [PMID: 19852088]
  3. Yi, C., Jia, G., Hou, G., Dai, Q., Zhang, W., Zheng, G., Jian, X., Yang, C. G., Cui, Q., He, C.
    Iron-catalysed oxidation intermediates captured in a DNA repair dioxygenase.
    Nature 468: 330-333 (2010). [PMID: 21068844]

[EC 1.14.11.33 created 2011]