EC 1.14.11.26 - Deacetoxycephalosporin-C hydroxylase

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IntEnz Enzyme Nomenclature
EC 1.14.11.26

Names

Accepted name:
deacetoxycephalosporin-C hydroxylase
Other names:
deacetylcephalosporin C synthase
3'-methylcephem hydroxylase
DACS
DAOC hydroxylase
deacetoxycephalosporin C hydroxylase
Systematic name:
deacetoxycephalosporin-C,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)

Reaction

Cofactor

Comments:

Requires iron(II). The enzyme can also use 3-exomethylenecephalosporin C as a substrate to form deacetoxycephalosporin C, although more slowly [2]. In Acremonium chrysogenum, the enzyme forms part of a bifunctional protein along with EC 1.14.20.1, deactoxycephalosporin-C synthase. It is a separate enzyme in Streptomyces clavuligerus.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00165 , PROSITE:PDOC51471
Structural data: CSA , EC2PDB
Gene Ontology: GO:0045442
UniProtKB/Swiss-Prot:

References

  1. Dotzlaf, J.E. and Yeh, W.-K.
    Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium.
    J. Bacteriol. 169: 1611-1618 (1987). [PMID: 3558321]
  2. Baker, B.J., Dotzlaf, J.E. and Yeh, W.-K.
    Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication.
    J. Biol. Chem. 266: 5087-5093 (1991). [PMID: 2002049]
  3. Coque, J.J.R., Enguita, F.J., Cardoza, R.E., Martín, J.F. and Liras, P.
    Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase.
    Appl. Microbiol. Biotechnol. 44: 605-609 (1996). [PMID: 8703431]
  4. Ghag, S.K., Brems, D.N., Hassell, T.C. and Yeh, W.-K.
    Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli.
    Biotechnol. Appl. Biochem. 24: 109-119 (1996). [PMID: 8865604]
  5. Lloyd, M.D., Lipscomb, S.J., Hewitson, K.S., Hensgens, C.M.H., Baldwin, J.E. and Schofield, C.J.
    Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase.
    J. Biol. Chem. 279: 15420-15426 (2004). [PMID: 14734549]
  6. Wu, X.-B., Fan, K.-Q., Wang, Q.-H. and Yang, K.-Q.
    C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs.
    FEMS Microbiol. Lett. 246: 103-110 (2005). [PMID: 15869968]
  7. Martín, J.F., Gutiérrez, S., Fernández, F.J., Velasco, J., Fierro, F., Marcos, A.T. and Kosalkova, K.
    Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins.
    Antonie Van Leeuwenhoek 65: 227-243 (1994). [PMID: 7847890]

[EC 1.14.11.26 created 2005]