EC 1.13.11.51 - 9-cis-epoxycarotenoid dioxygenase

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IntEnz Enzyme Nomenclature
EC 1.13.11.51

Names

Accepted name:
9-cis-epoxycarotenoid dioxygenase
Other names:
nine-cis-epoxycarotenoid dioxygenase
NCED
AtNCED3
PvNCED1
VP14
Systematic name:
9-cis-epoxycarotenoid 11,12-dioxygenase

Reactions

Cofactor

Comments:

Requires iron(II). Acts on 9-cis-violaxanthin and 9'-cis-neoxanthin but not on the all-trans isomers [2,3]. In vitro, it will cleave 9-cis-zeaxanthin. Catalyses the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress. The other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic aldehyde oxidase) and EC 1.14.13.93 [(+)-abscisic acid 8'-hydroxylase].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0045549
UniProtKB/Swiss-Prot: (15) [show] [UniProt]

References

  1. Schwartz, S.H., Tan, B.C., Gage, D.A., Zeevaart, J.A.D. and McCarty, D.R.
    Specific oxidative cleavage of carotenoids by VP14 of maize.
    Science 276 : 1872-1874 (1997). [PMID: 9188535]
  2. Tan, B.C., Schwartz, S.H., Zeevaart, J.A.D. and McCarty, D.R.
    Genetic control of abscisic acid biosynthesis in maize.
    Proc. Natl. Acad. Sci. USA 94 : 12235-12240 (1997). [PMID: 9342392]
  3. Qin, X. and Zeevaart, J.A.D.
    The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.
    Proc. Natl. Acad. Sci. USA 96 : 15354-15361 (2000). [PMID: 10611388]
  4. Thompson, A.J., Jackson, A.C., Symonds, R.C., Mulholland, B.J., Dadswell, A.R., Blake, P.S., Burbidge, A. and Taylor, I.B.
    Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.
    Plant J. 23 : 363-374 (2000). [PMID: 10929129]
  5. Iuchi, S., Kobayashi, M., Taji, T., Naramoto, M., Seki, M., Kato, T., Tabata, S., Kakubari, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K.
    Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.
    Plant J. 27 : 325-333 (2001). [PMID: 11532178]
  6. Iuchi, S., Kobayashi, M., Taji, T., Naramoto, M., Seki, M., Kato, T., Tabata, S., Kakubari, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K.
    Erratum report. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.
    Plant J. 30 : 611 (2002). [PMID: 11532178]

[EC 1.13.11.51 created 2005]