EC 1 - Oxidoreductases
EC 1.13 - Acting on single donors with incorporation of molecular oxygen (oxygenases). The oxygen incorporated need not be derived from O2
EC 1.13.11 - With incorporation of two atoms of oxygen
EC 1.13.11.51 - 9-cis-epoxycarotenoid dioxygenase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 1.13.11.51
Names
Accepted name:
9-cis-epoxycarotenoid dioxygenase
Other
names:
nine-cis-epoxycarotenoid dioxygenase
NCED
AtNCED3
PvNCED1
VP14
NCED
AtNCED3
PvNCED1
VP14
Systematic name:
9-cis-epoxycarotenoid 11,12-dioxygenase
Reactions
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23328 [IUBMB]a 9-cis-epoxycarotenoidName origin: UniProt - CHECKED (C)Formula: C29H39O2R
Charge: 0ChEBI compound status: CHECKED (C)O2Name origin: UniProt - CHECKED (C)Formula: O2
Charge: 0ChEBI compound status: CHECKED (C)=2-cis,4-trans-xanthoxinName origin: UniProt - CHECKED (C)Formula: C15H22O3
Charge: 0ChEBI compound status: CHECKED (C) -
16541 [IUBMB]9-cis-violaxanthinName origin: UniProt - CHECKED (C)Formula: C40H56O4
Charge: 0ChEBI compound status: CHECKED (C)O2Name origin: UniProt - CHECKED (C)Formula: O2
Charge: 0ChEBI compound status: CHECKED (C)=(3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-alName origin: UniProt - CHECKED (C)Formula: C25H34O3
Charge: 0ChEBI compound status: CHECKED (C) -
19677 [IUBMB]9'-cis-neoxanthinName origin: UniProt - CHECKED (C)Formula: C40H56O4
Charge: 0ChEBI compound status: CHECKED (C)O2Name origin: UniProt - CHECKED (C)Formula: O2
Charge: 0ChEBI compound status: CHECKED (C)=(3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-alName origin: UniProt - CHECKED (C)Formula: C25H34O3
Charge: 0ChEBI compound status: CHECKED (C)
Cofactor
Comments:
Requires iron(II). Acts on 9-cis-violaxanthin and 9'-cis-neoxanthin but not on the all-trans isomers [2,3]. In vitro, it will cleave 9-cis-zeaxanthin. Catalyses the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress. The other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic aldehyde oxidase) and EC 1.14.13.93 [(+)-abscisic acid 8'-hydroxylase].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
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ExplorEnz
,
ENZYME@ExPASy
,
KEGG
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MetaCyc
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UniPathway
Gene Ontology:
GO:0045549
References
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Specific oxidative cleavage of carotenoids by VP14 of maize.Science 276 : 1872-1874 (1997). [PMID: 9188535]
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Genetic control of abscisic acid biosynthesis in maize.Proc. Natl. Acad. Sci. USA 94 : 12235-12240 (1997). [PMID: 9342392]
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The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.Proc. Natl. Acad. Sci. USA 96 : 15354-15361 (2000). [PMID: 10611388]
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Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.Plant J. 23 : 363-374 (2000). [PMID: 10929129]
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Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.Plant J. 27 : 325-333 (2001). [PMID: 11532178]
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Erratum report. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.Plant J. 30 : 611 (2002). [PMID: 11532178]
[EC 1.13.11.51 created 2005]