EC - Protocatechuate 3,4-dioxygenase

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IntEnz Enzyme Nomenclature


Accepted name:
protocatechuate 3,4-dioxygenase
Other names:
protocatechuate oxygenase
protocatechuic 3,4-dioxygenase
protocatechuic 3,4-oxygenase
protocatechuic acid oxidase
Systematic name:
protocatechuate:oxygen 3,4-oxidoreductase (decyclizing)




Requires Fe3+. The enzyme, which participates in the degradation of aromatic compounds, catalyses the intradiol addition of both oxygen atoms from molecular oxygen, resulting in ortho-cleavage of the aromatic ring. The type of cleavage leads to mineralization via the intermediate 3-oxoadipate.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Protein domains and families: PROSITE:PDOC00079
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018578
CAS Registry Number: 9029-47-4


  1. Fujisawa, H. and Hayaishi, O.
    Protocatechuate 3,4-dioxygenase. I. Crystallization and characterization.
    J. Biol. Chem. 243 : 2673-2681 (1968). [PMID: 4967959]
  2. Gross, S.R., Gafford, R.D. and Tatum, E.L.
    The metabolism of protocatechuic acid by Neurospora.
    J. Biol. Chem. 219 : 781-796 (1956). [PMID: 13319299]
  3. Stanier, R.Y. and Ingraham, J.L.
    Protocatechuic acid oxidase.
    J. Biol. Chem. 210 : 799-820 (1954). [PMID: 13211618]

[EC created 1961 as EC, transferred 1965 to EC, transferred 1972 to EC]