EC - Coenzyme F420 hydrogenase

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IntEnz Enzyme Nomenclature


Accepted name:
coenzyme F420 hydrogenase
Other names:
8-hydroxy-5-deazaflavin-reducing hydrogenase
F420-reducing hydrogenase
coenzyme F420-dependent hydrogenase
Systematic name:
hydrogen:coenzyme F420 oxidoreductase




An iron-sulfur flavoprotein (FAD) containing nickel. The enzyme from some sources contains selenocysteine. The enzyme also reduces the riboflavin analogue of F420, flavins and methylviologen, but to a lesser extent. The hydrogen acceptor coenzyme F420 is a deazaflavin derivative.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050454
CAS Registry Number: 9027-05-8
UniProtKB/Swiss-Prot: (12) [show] [UniProt]


  1. Adams, M.W.W., Mortenson, L.E. and Chen, J.-S.
    Biochim. Biophys. Acta 594: 105-176 (1981). [PMID: 6786341]
  2. Yamazaki, S.
    A selenium-containing hydrogenase from Methanococcus vannielii. Identification of the selenium moiety as a selenocysteine residue.
    J. Biol. Chem. 257: 7926-7929 (1982). [PMID: 6211447]
  3. Fox, J.A., Livingston, D.J., Orme-Johnson, W.H. and Walsh, C.T.
    8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization.
    Biochemistry 26: 4219-4228 (1987). [PMID: 3663585]
  4. Muth, E., Morschel, E. and Klein, A.
    Purification and characterization of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from the archaebacterium Methanococcus voltae.
    Eur. J. Biochem. 169: 571-577 (1987). [PMID: 3121317]
  5. Baron, S.F. and Ferry, J.G.
    Purification and properties of the membrane-associated coenzyme F420-reducing hydrogenase from Methanobacterium formicicum.
    J. Bacteriol. 171: 3846-3853 (1989). [PMID: 2738024]

[EC created 1989 as EC, transferred 2002 to EC]