IntEnz

EC 1.12.1.3 - Hydrogen dehydrogenase (NADP⁺)

IntEnz Enzyme Nomenclature
EC 1.12.1.3

Names

Reaction

• 18637 [IUBMB]
  H₂

  H2

  Name origin: UniProt - CHECKED (C)

  CHEBI:18276

  Formula: H2
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  NADP⁺

  NADP(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:58349

  Formula: C21H25N7O17P3
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  =

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  NADPH

  NADPH

  Name origin: UniProt - CHECKED (C)

  CHEBI:57783

  Formula: C21H26N7O17P3
  Charge: -4

  ChEBI compound status: CHECKED (C)

  

Cofactors

• Fe cation
• Ni²⁺
• iron-sulfur

Comments:

The protein from the bacterium Desulfovibrio fructosovorans is an iron-sulfur protein that exclusively functions as a hydrogen dehydrogenase, while the enzyme from the archaeon Pyrococcus furiosus is a nickel, iron, iron-sulfur protein, that is part of a heterotetrameric complex where the α and δ subunits function as a hydrogenase while the β and γ subunits function as sulfur reductase (EC 1.12.98.4, sulfhydrogenase). Different from EC 1.12.1.5, hydrogen dehydrogenase [NAD(P)⁺].

Links to other databases

References

1. de Luca, G., de Philip, P., Rousset, M., Belaich, J.P. and Dermoun, Z.
   The NADP-reducing hydrogenase of Desulfovibrio fructosovorans: Evidence for a native complex with hydrogen-dependent methyl-viologen-reducing activity.
   Biochem. Biophys. Res. Commun. 248: 591-596 (1998). [PMID: 9703971]
2. Bryant, F. O., Adams, M. W.
   Characterization of hydrogenase from the hyperthermophilic archaebacterium, Pyrococcus furiosus.
   J. Biol. Chem. 264: 5070-5079 (1989). [PMID: 2538471]
3. Ma, K., Schicho, R. N., Kelly, R. M., Adams, M. W.
   Hydrogenase of the hyperthermophile Pyrococcus furiosus is an elemental sulfur reductase or sulfhydrogenase: evidence for a sulfur-reducing hydrogenase ancestor.
   Proc. Natl. Acad. Sci. U.S.A. 90: 5341-5344 (1993). [PMID: 8389482]
4. Ma, K., Zhou, Z.H. and Adams, M.W.
   Hydrogen production from pyruvate by enzymes purified from the hyperthermophilic archaeon, Pyrococcus furiosus: A key role for NADPH.
   FEMS Microbiol. Lett. 122: 245-250 (1994).
5. van Haaster, D. J., Silva, P. J., Hagedoorn, P. L., Jongejan, J. A., Hagen, W. R.
   Reinvestigation of the steady-state kinetics and physiological function of the soluble NiFe-hydrogenase I of Pyrococcus furiosus.
   J. Bacteriol. 190: 1584-1587 (2008). [PMID: 18156274]

[EC 1.12.1.3 created 2002, modified 2013]