EC - Versatile peroxidase

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IntEnz Enzyme Nomenclature


Accepted name:
versatile peroxidase
Other names:
hybrid peroxidase
polyvalent peroxidase
reactive-black-5:hydrogen-peroxide oxidoreductase
Systematic name:
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol:hydrogen-peroxide oxidoreductase




A hemoprotein. This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC, manganese peroxidase and EC, lignin peroxidase. Unlike these two enzymes, it is also able to oxidize phenols, hydroquinones and both low- and high-redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [2,4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00394
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052750


  1. Martínez, M.J., Ruiz-Dueñas, F. J., Guillén, F. and Martínez, A.T.
    Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii.
    Eur. J. Biochem. 237 : 424-432 (1996). [PMID: 8647081]
  2. Heinfling, A., Ruiz-Dueñas, F.J., Martínez, M.J., Bergbauer, M., Szewzyk, U. and Martínez, A.T.
    A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta.
    FEBS Lett. 428 : 141-146 (1998). [PMID: 9654123]
  3. Ruiz-Dueñas, F.J., Martínez, M.J. and Martínez, A.T.
    Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii.
    Mol. Microbiol. 31 : 223-235 (1999). [PMID: 9987124]
  4. Camarero, S., Sarkar, S., Ruiz-Dueñas, F.J., Martínez, M.J. and Martínez, A.T.
    Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites.
    J. Biol. Chem. 274 : 10324-10330 (1999). [PMID: 10187820]
  5. Ruiz-Dueñas, F.J., Martínez, M.J. and Martínez, A.T.
    Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn2+ and different aromatic substrates.
    Appl. Environ. Microbiol. 65 : 4705-4707 (1999). [PMID: 10508113]
  6. Camarero, S., Ruiz-Dueñas, F.J., Sarkar, S., Martínez, M.J. and Martínez, A.T.
    The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
    FEMS Microbiol. Lett. 191 : 37-43 (2000). [PMID: 11004397]
  7. Ruiz-Dueñas, F.J., Camarero, S., Pérez-Boada, M., Martínez, M.J. and Martínez, A.T.
    A new versatile peroxidase from Pleurotus.
    Biochem. Soc. Trans. 29 : 116-122 (2001). [PMID: 11356138]
  8. Banci, L., Camarero, S., Martínez, A.T., Martínez, M.J., Pérez-Boada, M., Pierattelli, R. and Ruiz-Dueñas, F.J.
    NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii.
    J. Biol. Inorg. Chem. 8 : 751-760 (2003). [PMID: 12884090]
  9. Pérez-Boada, M., Ruiz-Dueñas, F.J., Pogni, R., Basosi, R., Choinowski, T., Martínez, M.J., Piontek, K. and Martínez, A. T.
    Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways.
    J. Mol. Biol. 354 : 385-402 (2005). [PMID: 16246366]
  10. Caramelo, L., Martínez, M.J. and Martínez, A.T.
    A search for ligninolytic peroxidases in the fungus Pleurotus eryngii involving α-keto-γ-thiomethylbutyric acid and lignin model dimers
    Appl. Environ. Microbiol. 65 : 916-922 (1999). [PMID: 10049842]

[EC created 2006, modified 2016]