EC 1.1.99.29 - Pyranose dehydrogenase (acceptor)

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IntEnz Enzyme Nomenclature
EC 1.1.99.29

Names

Accepted name:
pyranose dehydrogenase (acceptor)
Other names:
PDH
pyranose dehydrogenase
pyranose-quinone oxidoreductase
quinone-dependent pyranose dehydrogenase
Systematic name:
pyranose:acceptor oxidoreductase

Reactions

Cofactor

Comments:

Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1→4-α- and 1→4-β-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. Sugars are oxidized in their pyranose but not in their furanose form.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033718
UniProtKB/Swiss-Prot:

References

  1. Volc, J., Kubtovǎ, E., Wood, D. and Daniel, G.
    Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus.
    Arch. Microbiol. 167: 119-125 (1997). [PMID: 9042751]
  2. Volc, J., Sedmera, P., Halada, P., Přikrylovǎ, V. and Daniel, G.
    C-2 and C-3 oxidation of D-Glc, and C-2 oxidation of D-Gal by pyranose dehydrogenase from Agaricus bisporus.
    Carbohydr. Res. 310: 151-156 (1998).
  3. Volc, J., Sedmera, P., Halada, P., Přikrylovǎ, V. and Haltrich, D.
    Double oxidation of D-xylose to D-glycero-pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.
    Carbohydr. Res. 329: 219-225 (2001). [PMID: 11086703]
  4. Volc, J., Kubtovǎ, E., Daniel, G., Sedmera, P. and Haltrich, D.
    Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes.
    Arch. Microbiol. 176: 178-186 (2001). [PMID: 11511865]
  5. Volc, J., Sedmera, P., Halada, P., Daniel, G., Přikrylovǎ, V. and Haltrich, D.
    C-3 oxidation of non-reducing sugars by a fungal pyranose dehydrogenase: spectral characterization.
    J. Mol. Catal., B Enzym. 17: 91-100 (2002).

[EC 1.1.99.29 created 2004]