EC 126.96.36.199 - Pyranose dehydrogenase (acceptor)
IntEnz Enzyme Nomenclature
quinone-dependent pyranose dehydrogenase
- (1) pyranose + acceptor = pyranos-2-ulose + reduced acceptor
- (2) pyranose + acceptor = pyranos-3-ulose + reduced acceptor
- (3) pyranose + acceptor = pyranos-2,3-diulose + reduced acceptor
- (4) a pyranoside + acceptor = a pyranosid-3-ulose + reduced acceptor
- (5) a pyranoside + acceptor = a pyranosid-3,4-diulose + reduced acceptor
Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 188.8.131.52, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1→4-α- and 1→4-β-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 184.108.40.206. Sugars are oxidized in their pyranose but not in their furanose form.
Links to other databases
Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus.Arch. Microbiol. 167: 119-125 (1997). [PMID: 9042751]
C-2 and C-3 oxidation of D-Glc, and C-2 oxidation of D-Gal by pyranose dehydrogenase from Agaricus bisporus.Carbohydr. Res. 310: 151-156 (1998).
Double oxidation of D-xylose to D-glycero-pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.Carbohydr. Res. 329: 219-225 (2001). [PMID: 11086703]
Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes.Arch. Microbiol. 176: 178-186 (2001). [PMID: 11511865]
C-3 oxidation of non-reducing sugars by a fungal pyranose dehydrogenase: spectral characterization.J. Mol. Catal., B Enzym. 17: 91-100 (2002).
[EC 220.127.116.11 created 2004]